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• W2161036639 abstract "In vitro transcription reactions were performed with isolated ventricular nuclei of adult rats to investigate whether increased mRNA levels of the inhibitory G protein alpha-subunit Gi alpha-2 after prolonged in vivo stimulation with the beta-adrenoceptor agonist isoprenaline are caused by increased transcription. Rats were treated by a 4-day subcutaneous infusion of isoprenaline (2.4 mg/kg per day) or 0.9% NaCl as control. To avoid the influence of developmental expression patterns, adult rats were chosen for all experiments. Signals for Gi alpha-2 and the stimulatory G protein alpha-subunit Gs alpha were specific and due to hybridization of nascent mRNA transcripts. In the isoprenaline group the transcriptional activity of Gi alpha-2 gene increased to 140% of the control value, whereas gene specific hybridization for Gs alpha remained unchanged. These results show that increased Gi alpha-2 mRNA levels after stimulation with isoprenaline are at least partially caused by enhanced transcription of Gi alpha-2 mRNA." @default.
• W2161036639 created "2016-06-24" @default.
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• W2161036639 date "1993-03-01" @default.
• W2161036639 modified "2023-09-23" @default.
• W2161036639 title "Isoprenaline stimulates gene transcription of the inhibitory G protein alpha-subunit Gi alpha-2 in rat heart." @default.
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• W2161036639 doi "https://doi.org/10.1161/01.res.72.3.696" @default.
• W2161036639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8431992" @default.
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