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• W2161088046 abstract "In the present study we investigated the effect of transient ischemia (13 and 17 min) followed by 1h and 3h of reperfusion on the Ca2+-dependent nitric oxide synthase (NOS) activity. The enzyme activity was measured by the conversion of 14[C]arginine to 14[C]citrulline and determined in the dorsal horn, intermediate zone and ventral horn of the gray matter and in the dorsal, lateral and ventral columns of the white matter of the rabbit spinal cord. Transient ischemia induced by infrarenal balloon occlusion of the abdominal aorta for 13 min was not strong enough to cause significant changes in the catalytic NOS activity, although the experimental animals tended to show a mild deficit in hindlimb movement. The increase of enzyme activity was significant in the dorsal horn, dorsal and lateral column after 17 min ischemia. During subsequent reperfusions catalytic NOS activity undergoes a permanent increase in the dorsal horn and a transient increase in the intermediate zone. The neurological impairment of the hindlimbs, characterized by a partial or total paraplegia after 17 min ischemia and subsequent reperfusions may reflect differences in the integrity of spinal interneurons in the intermediate zone, i.e., the region being highly sensitive to transient spinal cord ischemia. Our results indicate that catalytic NOS activity may be regionally and temporally affected due to an ischemic insult and following subsequent reperfusions." @default.
• W2161088046 created "2016-06-24" @default.
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• W2161088046 date "2004-01-01" @default.
• W2161088046 modified "2023-09-27" @default.
• W2161088046 title "Transient ischemia mediates dissimilarities in nitric oxide synthase activity in the spinal cord regions" @default.
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