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• W2161116308 abstract "Recently, we presented evidence for conventional, strand-coupled replication of mammalian mitochondrial DNA. Partially single-stranded replication intermediates detected in the same DNA preparations were assumed to derive from the previously described, strand-asymmetric mode of mitochondrial DNA replication. Here, we show that bona fide replication intermediates from highly purified mitochondria are essentially duplex throughout their length, but contain widespread regions of RNA:DNA hybrid, as a result of the incorporation of ribonucleotides on the light strand which are subsequently converted to DNA. Ribonucleotide-rich regions can be degraded to generate partially single-stranded molecules by RNase H treatment in vitro or during DNA extraction from crude mitochondria. Mammalian mitochondrial DNA replication thus proceeds mainly, or exclusively, by a strand-coupled mechanism." @default.
• W2161116308 created "2016-06-24" @default.
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• W2161116308 date "2002-11-01" @default.
• W2161116308 modified "2023-10-17" @default.
• W2161116308 title "Biased Incorporation of Ribonucleotides on the Mitochondrial L-Strand Accounts for Apparent Strand-Asymmetric DNA Replication" @default.
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• W2161116308 doi "https://doi.org/10.1016/s0092-8674(02)01075-9" @default.
• W2161116308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12437923" @default.
• W2161116308 hasPublicationYear "2002" @default.
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