FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2161286004> ?p ?o ?g. }

• W2161286004 endingPage "754" @default.
• W2161286004 startingPage "742" @default.
• W2161286004 abstract "The regional differentiation of the sperm surface has been studied with the aid of a novel covalent labeling technique that permits concurrent cytological, biochemical, and immunological analyses. For these studies isothiocyanate derivatives of fluorescein (FITC) and diiodofluorescein (IFC) were employed: the latter can be prepared with radioiodine to high specific activity (125IFC) and is an impermeant reagent for the erythrocyte surface. Sperm of sea urchin (Strongylocentrotus purpuratus), medaka )Oryzias latipes), and golden hamster bind the fluorescent chromophores with a nonuniform distribution, most of the fluorescence being associated with the midpiece. The radioactive derivative 125IFC permits an analysis of the proteins that are responsible for most of the binding. Additionally, 125 IFC-labeled sperm are capable of fertilizing eggs, as assessed by autoradiography. That IFC labels the surface of the sperm was inferred from the following: (a) the labeling of the surfaces of other cells by fluorescein isothiocyanate and its derivatives; (b) the agglutination of labeled sperm by antibodies directed against IFC; (c) the use of peroxidase-dependent immunocytochemical reaction using anti-IFC antibodies, with analysis by electron microscopy; and (d) extraction of labeled sea urchin sperm with Triton X-100 under conditions that preferentially solubilize the plasma membrane. The antiserum directed against IFC was used to isolate the labeled surface components from Triton X-100 extracts of whole sperm, by immunoprecipitation, with Staphylococcus-A protein serving as a coprecipitant. The results support previous data showing that the sperm surface is a heterogeneous mosaic of restricted domains, one notable zone being the midpiece, where common molecular properties may be shared by sperm with distinctly different morphologies. In addition, IFC-mediated covalent alteration of specific cell surface proteins may be used to label, to identify, and, with the use of anti-IFC antibodies, to isolate such proteins from other cellular constituents." @default.
• W2161286004 created "2016-06-24" @default.
• W2161286004 creator A5023705033 @default.
• W2161286004 creator A5064863646 @default.
• W2161286004 creator A5066100249 @default.
• W2161286004 date "1979-09-01" @default.
• W2161286004 modified "2023-09-23" @default.
• W2161286004 title "Regional differentiation of the sperm surface as studied with 125I-diiodofluorescein isothiocyanate, an impermeant reagent that allows isolation of the labeled components." @default.
• W2161286004 cites W1493567759 @default.
• W2161286004 cites W1524789263 @default.
• W2161286004 cites W1570776822 @default.
• W2161286004 cites W1978069201 @default.
• W2161286004 cites W1986875092 @default.
• W2161286004 cites W1993290689 @default.
• W2161286004 cites W1994996867 @default.
• W2161286004 cites W1995135269 @default.
• W2161286004 cites W1996269001 @default.
• W2161286004 cites W2004617962 @default.
• W2161286004 cites W2005101090 @default.
• W2161286004 cites W2006135603 @default.
• W2161286004 cites W2006387884 @default.
• W2161286004 cites W2009703524 @default.
• W2161286004 cites W2020831883 @default.
• W2161286004 cites W2023209136 @default.
• W2161286004 cites W2026569489 @default.
• W2161286004 cites W2028472208 @default.
• W2161286004 cites W2047234498 @default.
• W2161286004 cites W2054252027 @default.
• W2161286004 cites W2056410257 @default.
• W2161286004 cites W2058952400 @default.
• W2161286004 cites W2087029289 @default.
• W2161286004 cites W2092786730 @default.
• W2161286004 cites W2133898967 @default.
• W2161286004 cites W2144737343 @default.
• W2161286004 cites W2284702808 @default.
• W2161286004 cites W2290020893 @default.
• W2161286004 cites W2410442028 @default.
• W2161286004 cites W947302755 @default.
• W2161286004 doi "https://doi.org/10.1083/jcb.82.3.742" @default.
• W2161286004 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2110495" @default.
• W2161286004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/511933" @default.
• W2161286004 hasPublicationYear "1979" @default.
• W2161286004 type Work @default.
• W2161286004 sameAs 2161286004 @default.
• W2161286004 citedByCount "50" @default.
• W2161286004 countsByYear W21612860042015 @default.
• W2161286004 crossrefType "journal-article" @default.
• W2161286004 hasAuthorship W2161286004A5023705033 @default.
• W2161286004 hasAuthorship W2161286004A5064863646 @default.
• W2161286004 hasAuthorship W2161286004A5066100249 @default.
• W2161286004 hasBestOaLocation W21612860041 @default.
• W2161286004 hasConcept C121332964 @default.
• W2161286004 hasConcept C153911025 @default.
• W2161286004 hasConcept C159654299 @default.
• W2161286004 hasConcept C203014093 @default.
• W2161286004 hasConcept C2776908312 @default.
• W2161286004 hasConcept C2777019933 @default.
• W2161286004 hasConcept C2777569064 @default.
• W2161286004 hasConcept C2778015030 @default.
• W2161286004 hasConcept C2778575167 @default.
• W2161286004 hasConcept C2781087480 @default.
• W2161286004 hasConcept C55493867 @default.
• W2161286004 hasConcept C59822182 @default.
• W2161286004 hasConcept C62520636 @default.
• W2161286004 hasConcept C86803240 @default.
• W2161286004 hasConcept C91523082 @default.
• W2161286004 hasConcept C91881484 @default.
• W2161286004 hasConcept C95444343 @default.
• W2161286004 hasConceptScore W2161286004C121332964 @default.
• W2161286004 hasConceptScore W2161286004C153911025 @default.
• W2161286004 hasConceptScore W2161286004C159654299 @default.
• W2161286004 hasConceptScore W2161286004C203014093 @default.
• W2161286004 hasConceptScore W2161286004C2776908312 @default.
• W2161286004 hasConceptScore W2161286004C2777019933 @default.
• W2161286004 hasConceptScore W2161286004C2777569064 @default.
• W2161286004 hasConceptScore W2161286004C2778015030 @default.
• W2161286004 hasConceptScore W2161286004C2778575167 @default.
• W2161286004 hasConceptScore W2161286004C2781087480 @default.
• W2161286004 hasConceptScore W2161286004C55493867 @default.
• W2161286004 hasConceptScore W2161286004C59822182 @default.
• W2161286004 hasConceptScore W2161286004C62520636 @default.
• W2161286004 hasConceptScore W2161286004C86803240 @default.
• W2161286004 hasConceptScore W2161286004C91523082 @default.
• W2161286004 hasConceptScore W2161286004C91881484 @default.
• W2161286004 hasConceptScore W2161286004C95444343 @default.
• W2161286004 hasIssue "3" @default.
• W2161286004 hasLocation W21612860041 @default.
• W2161286004 hasLocation W21612860042 @default.
• W2161286004 hasLocation W21612860043 @default.
• W2161286004 hasLocation W21612860044 @default.
• W2161286004 hasOpenAccess W2161286004 @default.
• W2161286004 hasPrimaryLocation W21612860041 @default.
• W2161286004 hasRelatedWork W1529439997 @default.
• W2161286004 hasRelatedWork W1909187229 @default.
• W2161286004 hasRelatedWork W2010720305 @default.
• W2161286004 hasRelatedWork W2066953347 @default.
• W2161286004 hasRelatedWork W2087295967 @default.
• W2161286004 hasRelatedWork W2091249846 @default.

• next