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- W2161349337 abstract "We describe the characterization of a chip-based platform (3D-KITChip) for the three-dimensional cultivation of cells under perfusion conditions via magnetic resonance imaging (MRI). Besides the chip, the microfluidic system is comprised of a bioreactor housing, a medium supply, a pump for generating active flow conditions as well as a gas mixing station. The closed circulation loop is ideally suited for a characterization via MRI since the small bioreactor setup with active perfusion, driven by the pump from outside the coils, not only is completely MRI-compatible but also can be transferred into the magnetic coil of an experimental animal scanner. We have found that the two halves of the chip inside the bioreactor are homogeneously perfused with cell culture medium both with and without cells inside the 3D-KITChip. In addition, the homogeneity of perfusion is nearly independent from the flow rates investigated in this study, and furthermore, the setup shows excellent washout characteristics after spiking with Gadolinium-DOTA which makes it an ideal candidate for drug screening purposes. We, therefore, conclude that the 3D-KITChip is well suited as a platform for high-density three-dimensional cell cultures, especially those requiring a defined medium flow and/or gas supply in a precisely controllable three dimensional environment, like stem cells. Wir beschreiben die Charakterisierung einer Chip-basierten Plattform (3D-KITChip) für die dreidimensionale Kultivierung von Zellen unter Perfusionsbedingungen mittels Magnetresonanz-Tomographie. Das mikrofluidische System setzt sich, neben dem Chip, aus dem Biroeaktorgehäuse, einer Mediumvorlage, einer Pumpe für die Generierung eines aktiven Flusses und einer Gasmischanlage zusammen. Das geschlossen perfundierte System ist ideal für die Charakterisierung mittels MRT geeignet, da der kleine Bioreaktoraufbau mit aktivem Fluss, der durch eine Pumpe außerhalb der Spulen erzeugt wird, nicht nur komplett MRT-kompatibel ist, sondern auch in den Spulenkern eines tierexperimentellen Scanners transferiert werden kann. Wir konnten zeigen, dass beide Chiphälften im Bioreaktor homogen mit Zellkulturmedium perfundiert werden, sowohl mit als auch ohne Zellen im 3D-KITChip. Darüber hinaus konnten wir zeigen, dass die Homogenität der Perfusion nahezu unabhängig von der verwendeten Flussrate ist und dass das System exzellente Wash-out-Eigenschaften nach dem Spiken mit Gadolinium-DOTA aufweist. Aus diesen Ergebnissen schließen wir, dass der 3D-KITChip eine hervorragende Plattform für die dreidimensionale Zellkultur darstellt, vor allem für solche Zellen, die einen definierten Fluss von Medium und/oder Gas für ihre Kultur benötigen, wie z.B. Stammzellen." @default.
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- W2161349337 date "2013-05-01" @default.
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- W2161349337 title "Characterization of a chip-based bioreactor for three-dimensional cell cultivation via Magnetic Resonance Imaging" @default.
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- W2161349337 doi "https://doi.org/10.1016/j.zemedi.2013.01.003" @default.
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