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- W2161431492 abstract "A fundamental goal in biology is to determine how cellular organization is coupled to function. To achieve this goal, a better understanding of organelle composition and structure is needed. Although visualization of cellular organelles using fluorescence or electron microscopy (EM) has become a common tool for the cell biologist, recent advances are providing a clearer picture of the cell than ever before. In particular, advanced light-microscopy techniques are achieving resolutions below the diffraction limit and EM tomography provides high-resolution three-dimensional (3D) images of cellular structures. The ability to perform both fluorescence and electron microscopy on the same sample (correlative light and electron microscopy, CLEM) makes it possible to identify where a fluorescently labeled protein is located with respect to organelle structures visualized by EM. Here, we review the current state of the art in 3D biological imaging techniques with a focus on recent advances in electron microscopy and fluorescence super-resolution techniques." @default.
- W2161431492 created "2016-06-24" @default.
- W2161431492 creator A5037618615 @default.
- W2161431492 creator A5045407180 @default.
- W2161431492 date "2012-01-01" @default.
- W2161431492 modified "2023-10-10" @default.
- W2161431492 title "Advances in high-resolution imaging – techniques for three-dimensional imaging of cellular structures" @default.
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- W2161431492 doi "https://doi.org/10.1242/jcs.090027" @default.
- W2161431492 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3706075" @default.
- W2161431492 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22685332" @default.
- W2161431492 hasPublicationYear "2012" @default.
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