Matches in SemOpenAlex for { <https://semopenalex.org/work/W2161531330> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2161531330 endingPage "14613" @default.
- W2161531330 startingPage "14607" @default.
- W2161531330 abstract "Charybdotoxin is a high-affinity specific inhibitor of the high-conductance Ca2+-activated K+ channel found in the plasma membranes of many vertebrate cell types. Using Ca2+-activated K+ channels reconstituted into planar lipid bilayer membranes as an assay, we have purified the toxin from the venom of the scorpion Leiurus quinquestriatus by a two-step procedure involving chromatofocusing on SP-Sephadex, followed by reversed-phase high-performance liquid chromatography. Charybdotoxin is shown to be a highly basic protein with a mass of 10 kDa. Under our standard assay conditions, the purified toxin inhibits the Ca2+-activated K+ channel with an apparent dissociation constant of 3.5 nM. The protein is unusually stable, with inhibitory potency being insensitive to boiling or exposure to organic solvents. The toxin's activity is sensitive to chymotrypsin treatment and to acylation of lysine groups. The protein may be radioiodinated without loss of activity." @default.
- W2161531330 created "2016-06-24" @default.
- W2161531330 creator A5008760207 @default.
- W2161531330 creator A5037327500 @default.
- W2161531330 creator A5089038228 @default.
- W2161531330 date "1986-11-01" @default.
- W2161531330 modified "2023-10-18" @default.
- W2161531330 title "Purification of charybdotoxin, a specific inhibitor of the high-conductance Ca2+-activated K+ channel." @default.
- W2161531330 cites W1419481270 @default.
- W2161531330 cites W1497344470 @default.
- W2161531330 cites W1501758717 @default.
- W2161531330 cites W1561943245 @default.
- W2161531330 cites W1975957247 @default.
- W2161531330 cites W1979287010 @default.
- W2161531330 cites W1982319725 @default.
- W2161531330 cites W1995787167 @default.
- W2161531330 cites W2014321075 @default.
- W2161531330 cites W2018155809 @default.
- W2161531330 cites W2033817793 @default.
- W2161531330 cites W2046517694 @default.
- W2161531330 cites W2050565630 @default.
- W2161531330 cites W2063598077 @default.
- W2161531330 cites W2067860787 @default.
- W2161531330 cites W2069523275 @default.
- W2161531330 cites W2080240343 @default.
- W2161531330 cites W2082304034 @default.
- W2161531330 cites W2084797262 @default.
- W2161531330 cites W2087298783 @default.
- W2161531330 cites W2101966660 @default.
- W2161531330 cites W2126948267 @default.
- W2161531330 cites W38484849 @default.
- W2161531330 doi "https://doi.org/10.1016/s0021-9258(18)66914-5" @default.
- W2161531330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2429958" @default.
- W2161531330 hasPublicationYear "1986" @default.
- W2161531330 type Work @default.
- W2161531330 sameAs 2161531330 @default.
- W2161531330 citedByCount "120" @default.
- W2161531330 countsByYear W21615313302012 @default.
- W2161531330 countsByYear W21615313302013 @default.
- W2161531330 countsByYear W21615313302014 @default.
- W2161531330 countsByYear W21615313302015 @default.
- W2161531330 countsByYear W21615313302017 @default.
- W2161531330 countsByYear W21615313302021 @default.
- W2161531330 crossrefType "journal-article" @default.
- W2161531330 hasAuthorship W2161531330A5008760207 @default.
- W2161531330 hasAuthorship W2161531330A5037327500 @default.
- W2161531330 hasAuthorship W2161531330A5089038228 @default.
- W2161531330 hasBestOaLocation W21615313301 @default.
- W2161531330 hasConcept C121332964 @default.
- W2161531330 hasConcept C121932024 @default.
- W2161531330 hasConcept C12554922 @default.
- W2161531330 hasConcept C127162648 @default.
- W2161531330 hasConcept C181911157 @default.
- W2161531330 hasConcept C185592680 @default.
- W2161531330 hasConcept C26873012 @default.
- W2161531330 hasConcept C2776919887 @default.
- W2161531330 hasConcept C2780419564 @default.
- W2161531330 hasConcept C31258907 @default.
- W2161531330 hasConcept C41008148 @default.
- W2161531330 hasConcept C55493867 @default.
- W2161531330 hasConcept C83743174 @default.
- W2161531330 hasConcept C86803240 @default.
- W2161531330 hasConcept C95444343 @default.
- W2161531330 hasConceptScore W2161531330C121332964 @default.
- W2161531330 hasConceptScore W2161531330C121932024 @default.
- W2161531330 hasConceptScore W2161531330C12554922 @default.
- W2161531330 hasConceptScore W2161531330C127162648 @default.
- W2161531330 hasConceptScore W2161531330C181911157 @default.
- W2161531330 hasConceptScore W2161531330C185592680 @default.
- W2161531330 hasConceptScore W2161531330C26873012 @default.
- W2161531330 hasConceptScore W2161531330C2776919887 @default.
- W2161531330 hasConceptScore W2161531330C2780419564 @default.
- W2161531330 hasConceptScore W2161531330C31258907 @default.
- W2161531330 hasConceptScore W2161531330C41008148 @default.
- W2161531330 hasConceptScore W2161531330C55493867 @default.
- W2161531330 hasConceptScore W2161531330C83743174 @default.
- W2161531330 hasConceptScore W2161531330C86803240 @default.
- W2161531330 hasConceptScore W2161531330C95444343 @default.
- W2161531330 hasIssue "31" @default.
- W2161531330 hasLocation W21615313301 @default.
- W2161531330 hasOpenAccess W2161531330 @default.
- W2161531330 hasPrimaryLocation W21615313301 @default.
- W2161531330 hasRelatedWork W1993802295 @default.
- W2161531330 hasRelatedWork W2005750547 @default.
- W2161531330 hasRelatedWork W2011675741 @default.
- W2161531330 hasRelatedWork W2035459073 @default.
- W2161531330 hasRelatedWork W2043762161 @default.
- W2161531330 hasRelatedWork W2045400413 @default.
- W2161531330 hasRelatedWork W2077739881 @default.
- W2161531330 hasRelatedWork W2115563064 @default.
- W2161531330 hasRelatedWork W2161661722 @default.
- W2161531330 hasRelatedWork W2419342829 @default.
- W2161531330 hasVolume "261" @default.
- W2161531330 isParatext "false" @default.
- W2161531330 isRetracted "false" @default.
- W2161531330 magId "2161531330" @default.
- W2161531330 workType "article" @default.