FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2161566797> ?p ?o ?g. }

• W2161566797 endingPage "1919" @default.
• W2161566797 startingPage "1913" @default.
• W2161566797 abstract "Recent studies have reported that mutant genomic cystic fibrosis (CF) transmembrane conductance regulator (CFTR) sequences can be corrected in transformed CF airway epithelial cell lines by targeted replacement with small fragments of DNA with wild-type sequence. To determine if the observed genotype modification following small fragment homologous replacement (SFHR) was limited to transformed CF cell lines, further studies were carried out in both transformed and non-transformed primary normal airway epithelial cells. The endogenous genotype of these normal cell lines was modified following liposome or dendrimer transfection using DNA fragments with ΔF508 CFTR sequence (488 nt, complementary single strands) designed to also contain a unique restriction enzyme cleavage site (XhoI). Replacement at the appropriate genomic locus by exogenous ΔF508 CFTR DNA and its expression as mRNA was demonstrated by PCR amplification of genomic DNA and mRNA-derived cDNA as well as XhoI digestion of the PCR products. These studies show that SFHR occurs in both transformed and non-transformed primary human airway epithelial cells and indicate that single base substitution (the silent mutation giving rise to the XhoI site) and deletion or insertion of at least three consecutive bases can be achieved in both normal and CF epithelial cells. Furthermore, these studies reiterate the potential of SFHR as a strategy for a number of gene targeting applications, such as site-specific mutagenesis, development of transgenic animals, development of isogenic cell lines and for gene therapy." @default.
• W2161566797 created "2016-06-24" @default.
• W2161566797 creator A5046074910 @default.
• W2161566797 creator A5051912802 @default.
• W2161566797 creator A5058904925 @default.
• W2161566797 creator A5077643720 @default.
• W2161566797 date "1998-11-01" @default.
• W2161566797 modified "2023-10-11" @default.
• W2161566797 title "Targeted replacement of normal and mutant CFTR sequences in human airway epithelial cells using DNA fragments" @default.
• W2161566797 cites W1590618726 @default.
• W2161566797 cites W1825482480 @default.
• W2161566797 cites W1969697265 @default.
• W2161566797 cites W1973919938 @default.
• W2161566797 cites W1989150670 @default.
• W2161566797 cites W1990991203 @default.
• W2161566797 cites W1991062831 @default.
• W2161566797 cites W2001337552 @default.
• W2161566797 cites W2004908650 @default.
• W2161566797 cites W2005644246 @default.
• W2161566797 cites W2006683789 @default.
• W2161566797 cites W2008661813 @default.
• W2161566797 cites W2016073646 @default.
• W2161566797 cites W2019364665 @default.
• W2161566797 cites W2019965747 @default.
• W2161566797 cites W2020101522 @default.
• W2161566797 cites W2020862993 @default.
• W2161566797 cites W2039599894 @default.
• W2161566797 cites W2044150368 @default.
• W2161566797 cites W2050734291 @default.
• W2161566797 cites W2053191459 @default.
• W2161566797 cites W2057940358 @default.
• W2161566797 cites W2067379696 @default.
• W2161566797 cites W2070122091 @default.
• W2161566797 cites W2085848981 @default.
• W2161566797 cites W2089642814 @default.
• W2161566797 cites W2090081965 @default.
• W2161566797 cites W2090541586 @default.
• W2161566797 cites W2091445108 @default.
• W2161566797 cites W2141365582 @default.
• W2161566797 cites W2156420984 @default.
• W2161566797 cites W2180906670 @default.
• W2161566797 cites W4256704256 @default.
• W2161566797 doi "https://doi.org/10.1093/hmg/7.12.1913" @default.
• W2161566797 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9811935" @default.
• W2161566797 hasPublicationYear "1998" @default.
• W2161566797 type Work @default.
• W2161566797 sameAs 2161566797 @default.
• W2161566797 citedByCount "105" @default.
• W2161566797 countsByYear W21615667972012 @default.
• W2161566797 countsByYear W21615667972013 @default.
• W2161566797 countsByYear W21615667972014 @default.
• W2161566797 countsByYear W21615667972015 @default.
• W2161566797 countsByYear W21615667972016 @default.
• W2161566797 countsByYear W21615667972017 @default.
• W2161566797 countsByYear W21615667972018 @default.
• W2161566797 countsByYear W21615667972019 @default.
• W2161566797 countsByYear W21615667972020 @default.
• W2161566797 countsByYear W21615667972022 @default.
• W2161566797 countsByYear W21615667972023 @default.
• W2161566797 crossrefType "journal-article" @default.
• W2161566797 hasAuthorship W2161566797A5046074910 @default.
• W2161566797 hasAuthorship W2161566797A5051912802 @default.
• W2161566797 hasAuthorship W2161566797A5058904925 @default.
• W2161566797 hasAuthorship W2161566797A5077643720 @default.
• W2161566797 hasBestOaLocation W21615667971 @default.
• W2161566797 hasConcept C104317684 @default.
• W2161566797 hasConcept C128319531 @default.
• W2161566797 hasConcept C130123407 @default.
• W2161566797 hasConcept C143065580 @default.
• W2161566797 hasConcept C153911025 @default.
• W2161566797 hasConcept C17757408 @default.
• W2161566797 hasConcept C187882448 @default.
• W2161566797 hasConcept C2778428886 @default.
• W2161566797 hasConcept C2778972215 @default.
• W2161566797 hasConcept C54009773 @default.
• W2161566797 hasConcept C54355233 @default.
• W2161566797 hasConcept C552990157 @default.
• W2161566797 hasConcept C86803240 @default.
• W2161566797 hasConceptScore W2161566797C104317684 @default.
• W2161566797 hasConceptScore W2161566797C128319531 @default.
• W2161566797 hasConceptScore W2161566797C130123407 @default.
• W2161566797 hasConceptScore W2161566797C143065580 @default.
• W2161566797 hasConceptScore W2161566797C153911025 @default.
• W2161566797 hasConceptScore W2161566797C17757408 @default.
• W2161566797 hasConceptScore W2161566797C187882448 @default.
• W2161566797 hasConceptScore W2161566797C2778428886 @default.
• W2161566797 hasConceptScore W2161566797C2778972215 @default.
• W2161566797 hasConceptScore W2161566797C54009773 @default.
• W2161566797 hasConceptScore W2161566797C54355233 @default.
• W2161566797 hasConceptScore W2161566797C552990157 @default.
• W2161566797 hasConceptScore W2161566797C86803240 @default.
• W2161566797 hasIssue "12" @default.
• W2161566797 hasLocation W21615667971 @default.
• W2161566797 hasLocation W21615667972 @default.
• W2161566797 hasOpenAccess W2161566797 @default.
• W2161566797 hasPrimaryLocation W21615667971 @default.
• W2161566797 hasRelatedWork W1547565926 @default.
• W2161566797 hasRelatedWork W1980481823 @default.

• next