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• W2161914201 endingPage "3314" @default.
• W2161914201 startingPage "3303" @default.
• W2161914201 abstract "ABSTRACT Moorella thermoacetica can grow with H 2 and CO 2 , forming acetic acid from 2 CO 2 via the Wood-Ljungdahl pathway. All enzymes involved in this pathway have been characterized to date, except for methylenetetrahydrofolate reductase (MetF). We report here that the M. thermoacetica gene that putatively encodes this enzyme, metF , is part of a transcription unit also containing the genes hdrCBA , mvhD , and metV . MetF copurified with the other five proteins encoded in the unit in a hexaheteromeric complex with an apparent molecular mass in the 320-kDa range. The 40-fold-enriched preparation contained per mg protein 3.1 nmol flavin adenine dinucleotide (FAD), 3.4 nmol flavin mononucleotide (FMN), and 110 nmol iron, almost as predicted from the primary structure of the six subunits. It catalyzed the reduction of methylenetetrahydrofolate with reduced benzyl viologen but not with NAD(P)H in either the absence or presence of oxidized ferredoxin. It also catalyzed the reversible reduction of benzyl viologen with NADH (diaphorase activity). Heterologous expression of the metF gene in Escherichia coli revealed that the subunit MetF contains one FMN rather than FAD. MetF exhibited 70-fold-higher methylenetetrahydrofolate reductase activity with benzyl viologen when produced together with MetV, which in part shows sequence similarity to MetF. Heterologously produced HdrA contained 2 FADs and had NAD-specific diaphorase activity. Our results suggested that the physiological electron donor for methylenetetrahydrofolate reduction in M. thermoacetica is NADH and that the exergonic reduction of methylenetetrahydrofolate with NADH is coupled via flavin-based electron bifurcation with the endergonic reduction of an electron acceptor, whose identity remains unknown." @default.
• W2161914201 created "2016-06-24" @default.
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• W2161914201 creator A5077679908 @default.
• W2161914201 creator A5088235237 @default.
• W2161914201 date "2014-09-15" @default.
• W2161914201 modified "2023-10-16" @default.
• W2161914201 title "Evidence for a Hexaheteromeric Methylenetetrahydrofolate Reductase in Moorella thermoacetica" @default.
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• W2161914201 doi "https://doi.org/10.1128/jb.01839-14" @default.
• W2161914201 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4135698" @default.
• W2161914201 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25002540" @default.
• W2161914201 hasPublicationYear "2014" @default.
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