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• W2161993072 abstract "Abstract We have previously observed that a 6-day exposure of human pancreatic islets to a combination of cytokines (interleukin-1β 50 U/ml + tumour necrosis factor-α 1000 U/ml + interferon-γ 1000 U/ml) severely impairs β-cell functions. In the present study, we examined whether this condition affects DNA integrity and viability of human islet cells. Cells were studied after 3, 6, and 9 days of cytokine treatment by both single cell gel electrophoresis (the “comet assay,” a sensitive method for detection of DNA strand breaks) and by a cytotoxicity assay using the DNA binding dyes Hoechst 33342 and propidium iodide as indices for the number of viable, necrotic, and apoptotic cells. Cytokine treatment for 6 and 9 days resulted in a 50% increase in comet length (P &lt; 0.01 vs. controls), indicating DNA strand breaks, as well as in a significant increase in the number of apoptotic cells (P &lt; 0.02 vs. controls), but not in the number of necrotic cells. The arginine analogs NG-nitro-l-arginine and NG-monomethyl-l-arginine prevented nitric oxide formation by the cytokines but did not interfere with cytokine-induced DNA strand breaks and apoptosis. The present data suggest that prolonged (6–9 days) exposure of human pancreatic islets to a mixture of cytokines induces DNA strand breaks and cell death by apoptosis. These deleterious effects of cytokines appear to be independent of nitric oxide generation." @default.
• W2161993072 created "2016-06-24" @default.
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• W2161993072 date "1997-06-01" @default.
• W2161993072 modified "2023-10-16" @default.
• W2161993072 title "Cytokines Induce Deoxyribonucleic Acid Strand Breaks and Apoptosis in Human Pancreatic Islet Cells*" @default.
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• W2161993072 doi "https://doi.org/10.1210/endo.138.6.5204" @default.
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