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- W2162210654 startingPage "1341" @default.
- W2162210654 abstract "The regulatory factor Differentiation Inhibiting Activity/Leukaemia Inhibitory Factor (DIA/LIF) suppresses the differentiation of cultured embryonic stem (ES) cells. In the present study, it is shown that ES cell lines can be derived and maintained in the absence of feeder layers using medium supplemented with purified DIA/LIF. These cells can differentiate normally in vitro and in vivo and they retain the capacity for germ-line transmission. DIA/LIF therefore fulfils the essential function of feeders in the isolation of pluripotential stem cells." @default.
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- W2162210654 date "1990-12-01" @default.
- W2162210654 modified "2023-09-30" @default.
- W2162210654 title "Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity" @default.
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- W2162210654 doi "https://doi.org/10.1242/dev.110.4.1341" @default.
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