FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2162214141> ?p ?o ?g. }

• W2162214141 endingPage "84" @default.
• W2162214141 startingPage "876" @default.
• W2162214141 abstract "Calcitonin (CT) is a 32-amino-acid calciotropic peptide hormone which acts on target cells via a G protein-coupled seven-transmembrane receptor (CTR). In this study, we report the design, synthesis and characterization of four potent bioactive and photoreactive CT analogs, each of which contains a single benzophenone moiety inserted at different and discrete locations within the CT molecule. Replacement of all Lys residues in salmon CT (sCT) with Arg, followed by replacement of hydrophobic residues with a Lys(epsilon-p-benzoylbenzoyl) residue [Lys(epsilon-pBz2)] was found to preserve high biological activity. We substituted Val8, Leu16 and Leu19 by Lys(epsilon-pBz2), and acylated the N-terminus by a pBz2 moiety, thus distributing the photoaffinity moiety in the different analogs across a large portion of the CT sequence. With both transfected and endogenous CTRs from several species, all four benzophenone-containing analogs were shown to be virtually indistinguishable from the parent sCT analog in both receptor binding properties and stimulation of cAMP accumulation. Upon photolysis, in the presence of CTR, the radioiodinated photoreactive CT analog ([Arg11,18,Lys19(epsilon-pBz2)]sCT (K19)) covalently labels a membrane component of approximately 70 kDa. Receptor cross-linking is inhibited specifically in the presence of excess sCT. We also examined the interaction of these CT analogs with a hemagglutinin (HA) epitope-tagged CTR. The HA-CTR displayed CT binding and CT-dependent cAMP stimulation identical with native CTR. Both K19 and another bioactive analog (-Arg11,18, Lys8(epsilon-pBz2)]sCT (K8)) specifically photoaffinity cross-link to the HA-CTR. These benzophenone-containing CT analogs should facilitate studies of hormone-receptor interactions and allow the direct identification of a CT binding domain(s) within the receptor by the analysis of photochemically cross-linked conjugates." @default.
• W2162214141 created "2016-06-24" @default.
• W2162214141 creator A5007363699 @default.
• W2162214141 creator A5041873030 @default.
• W2162214141 creator A5042599014 @default.
• W2162214141 creator A5061211092 @default.
• W2162214141 creator A5065929346 @default.
• W2162214141 creator A5070219874 @default.
• W2162214141 creator A5080984126 @default.
• W2162214141 creator A5085901971 @default.
• W2162214141 date "1997-11-01" @default.
• W2162214141 modified "2023-09-27" @default.
• W2162214141 title "Design, synthesis and utility of novel benzophenone-containing calcitonin analogs for photoaffinity labeling the calcitonin receptor." @default.
• W2162214141 cites W1523989569 @default.
• W2162214141 cites W1628616828 @default.
• W2162214141 cites W1839474149 @default.
• W2162214141 cites W1966819735 @default.
• W2162214141 cites W1969080702 @default.
• W2162214141 cites W1971753539 @default.
• W2162214141 cites W1975214384 @default.
• W2162214141 cites W1981293067 @default.
• W2162214141 cites W1982749890 @default.
• W2162214141 cites W1989809710 @default.
• W2162214141 cites W1992553937 @default.
• W2162214141 cites W1995044901 @default.
• W2162214141 cites W1995853487 @default.
• W2162214141 cites W1999968337 @default.
• W2162214141 cites W2000867808 @default.
• W2162214141 cites W2009411531 @default.
• W2162214141 cites W2015722943 @default.
• W2162214141 cites W2017467085 @default.
• W2162214141 cites W2021769884 @default.
• W2162214141 cites W2025663580 @default.
• W2162214141 cites W2026018179 @default.
• W2162214141 cites W2028318691 @default.
• W2162214141 cites W2028616850 @default.
• W2162214141 cites W2038516932 @default.
• W2162214141 cites W2040067193 @default.
• W2162214141 cites W2043610823 @default.
• W2162214141 cites W2057682100 @default.
• W2162214141 cites W2070929561 @default.
• W2162214141 cites W2076913495 @default.
• W2162214141 cites W2080044485 @default.
• W2162214141 cites W2082311853 @default.
• W2162214141 cites W2084913828 @default.
• W2162214141 cites W2095070863 @default.
• W2162214141 cites W2096424250 @default.
• W2162214141 cites W2112806290 @default.
• W2162214141 cites W2124441769 @default.
• W2162214141 cites W2125046901 @default.
• W2162214141 cites W2138007390 @default.
• W2162214141 cites W2144617625 @default.
• W2162214141 cites W2152691788 @default.
• W2162214141 cites W2155221296 @default.
• W2162214141 cites W2157114424 @default.
• W2162214141 cites W2158992629 @default.
• W2162214141 cites W2166549790 @default.
• W2162214141 cites W3027873716 @default.
• W2162214141 cites W69504871 @default.
• W2162214141 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9353409" @default.
• W2162214141 hasPublicationYear "1997" @default.
• W2162214141 type Work @default.
• W2162214141 sameAs 2162214141 @default.
• W2162214141 citedByCount "2" @default.
• W2162214141 crossrefType "journal-article" @default.
• W2162214141 hasAuthorship W2162214141A5007363699 @default.
• W2162214141 hasAuthorship W2162214141A5041873030 @default.
• W2162214141 hasAuthorship W2162214141A5042599014 @default.
• W2162214141 hasAuthorship W2162214141A5061211092 @default.
• W2162214141 hasAuthorship W2162214141A5065929346 @default.
• W2162214141 hasAuthorship W2162214141A5070219874 @default.
• W2162214141 hasAuthorship W2162214141A5080984126 @default.
• W2162214141 hasAuthorship W2162214141A5085901971 @default.
• W2162214141 hasConcept C134018914 @default.
• W2162214141 hasConcept C13935069 @default.
• W2162214141 hasConcept C170493617 @default.
• W2162214141 hasConcept C185592680 @default.
• W2162214141 hasConcept C2776568683 @default.
• W2162214141 hasConcept C2778279444 @default.
• W2162214141 hasConcept C2778581200 @default.
• W2162214141 hasConcept C2779281246 @default.
• W2162214141 hasConcept C2779627488 @default.
• W2162214141 hasConcept C2781338088 @default.
• W2162214141 hasConcept C55493867 @default.
• W2162214141 hasConcept C71240020 @default.
• W2162214141 hasConcept C75473681 @default.
• W2162214141 hasConcept C86803240 @default.
• W2162214141 hasConceptScore W2162214141C134018914 @default.
• W2162214141 hasConceptScore W2162214141C13935069 @default.
• W2162214141 hasConceptScore W2162214141C170493617 @default.
• W2162214141 hasConceptScore W2162214141C185592680 @default.
• W2162214141 hasConceptScore W2162214141C2776568683 @default.
• W2162214141 hasConceptScore W2162214141C2778279444 @default.
• W2162214141 hasConceptScore W2162214141C2778581200 @default.
• W2162214141 hasConceptScore W2162214141C2779281246 @default.
• W2162214141 hasConceptScore W2162214141C2779627488 @default.
• W2162214141 hasConceptScore W2162214141C2781338088 @default.
• W2162214141 hasConceptScore W2162214141C55493867 @default.

• next