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- W2162219993 abstract "It has been generally assumed that the [alpha]-(1->4)-linked and [alpha]-(1->6)-branched glucans of starch are generated by the coordinated action of elongation (starch synthases) and branching enzymes. We have identified a novel Chlamydomonas locus (STA7) that when defective leads to a wipeout of starch and its replacement by a small amount of glycogen-like material. Our efforts to understand the enzymological basis of this phenotype have led us to determine the selective disappearance of an 88-kD starch hydrolytic activity. We further demonstrate that this enzyme is a debranching enzyme. Cleavage of the [alpha]-(1->6) linkage in a branched precursor of amylopectin (preamylopectin) has provided us with the ground rules for understanding starch biosynthesis in plants. Therefore, we propose that amylopectin clusters are synthesized by a discontinuous mechanism involving a highly specific glucan trimming mechanism." @default.
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- W2162219993 date "1996-08-01" @default.
- W2162219993 modified "2023-10-17" @default.
- W2162219993 title "Preamylopectin Processing: A Mandatory Step for Starch Biosynthesis in Plants." @default.
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- W2162219993 doi "https://doi.org/10.1105/tpc.8.8.1353" @default.
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