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- W2162248331 abstract "A new approach to the synthesis of oligonucleotides and oligonucleotide phosphorothioates in solution is described; it is based on H-phosphonate coupling [with bis(2-chlorophenyl) phosphorochloridate 22 as the coupling agent] at –40 °C, followed by in situ sulfur transfer involving either 2-(4-chlorophenylsulfanyl)isoindole-1,3(2H)-dione 23a or 4-[(2-cyanoethyl)sulfanyl]morpholine-3,5-dione 26. The yields of the coupling and sulfur transfer reactions are virtually quantitative and, following unblocking by previously reported procedures, very pure products (d[ApC], d[TpGpApC], d[TpGp(s)ApC], d[Gp(s)A] and d[Cp(s)Tp(s)Gp(s)A]) are obtained." @default.
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- W2162248331 date "1999-01-01" @default.
- W2162248331 modified "2023-10-13" @default.
- W2162248331 title "The H-phosphonate approach to the synthesis of oligonucleotides and their phosphorothioate analogues in solution" @default.
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- W2162248331 doi "https://doi.org/10.1039/a901301f" @default.
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