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- W2162287698 abstract "To establish a highly informative screening system for immunologically relevant genes (“immunoprinting”) we co-amplified via polymerase chain reaction (PCR) polymorphic exons plus adjacent intronic simple repetitive dinucleotide stretches in the T-cell receptor (Tcr)Vb6 and Major Histocompatibility Complex (MHC)-DRB loci in man and several ungulate species. In both gene families the basic structure of the simple repeat was found to be preserved for more than 70 × 106 years in all investigated species. The simple repeats exhibit extensive length variability. Distinct exon sequences are correlated with a defined repeat length and substructure. In addition, PCR and the oligonucleotides for typing were applicable to a broad range of species from different mammalian orders. Multiplex PCR of different members of the Tcr Vb6 family and MHC-DRB resulted in a complex pattern similar to an oligolocus fingerprint. Hence immunoprinting can be employed for searching for associations of immunologically relevant genes with diseases even across species barriers." @default.
- W2162287698 created "2016-06-24" @default.
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- W2162287698 date "1993-01-01" @default.
- W2162287698 modified "2023-09-27" @default.
- W2162287698 title "Towards covering immunological genes with highly informative markers: A trans-species approach" @default.
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- W2162287698 doi "https://doi.org/10.1007/978-3-0348-8583-6_9" @default.
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