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• W2162560305 abstract "Abstract Background Labeling whole Arabidopsis (Arabidopsis thaliana) plants to high enrichment with 13 C for proteomics and metabolomics applications would facilitate experimental approaches not possible by conventional methods. Such a system would use the plant's native capacity for carbon fixation to ubiquitously incorporate 13 C from 13 CO 2 gas. Because of the high cost of 13 CO 2 it is critical that the design conserve the labeled gas. Results A fully enclosed automated plant growth enclosure has been designed and assembled where the system simultaneously monitors humidity, temperature, pressure and 13 CO 2 concentration with continuous adjustment of humidity, pressure and 13 CO 2 levels controlled by a computer running LabView software. The enclosure is mounted on a movable cart for mobility among growth environments. Arabidopsis was grown in the enclosure for up to 8 weeks and obtained on average >95 atom% enrichment for small metabolites, such as amino acids and >91 atom% for large metabolites, including proteins and peptides. Conclusion The capability of this labeling system for isotope dilution experiments was demonstrated by evaluation of amino acid turnover using GC-MS as well as protein turnover using LC-MS/MS. Because this 'open source' Arabidopsis 13 C-labeling growth environment was built using readily available materials and software, it can be adapted easily to accommodate many different experimental designs." @default.
• W2162560305 created "2016-06-24" @default.
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• W2162560305 date "2011-02-10" @default.
• W2162560305 modified "2023-10-09" @default.
• W2162560305 title "An automated growth enclosure for metabolic labeling of Arabidopsis thaliana with 13C-carbon dioxide - an in vivo labeling system for proteomics and metabolomics research" @default.
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• W2162560305 doi "https://doi.org/10.1186/1477-5956-9-9" @default.
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