Matches in SemOpenAlex for { <https://semopenalex.org/work/W2162628872> ?p ?o ?g. }
- W2162628872 endingPage "490" @default.
- W2162628872 startingPage "483" @default.
- W2162628872 abstract "Twenty-four Scottish Blackface ewes (mean weight 50.0 ± 0.1 kg with ovulation rate 1.3 ± 0.1) were randomly divided into 4 groups of 6 animals. Under general anesthesia, following the collection of a timed sample of ovarian venous blood, the ovaries of these animals were collected either on Day 10 of the luteal phase or 12, 24, and 48 h after a luteolytic dose of a prostaglandin (PG) F2α analogue (cloprostenol 100 µ i.m.) administered on Day 10. All follicles greater than 3 mm were dissected from the ovaries and incubated in Medium 199 (M199) at 37°C for 2 h, following which the granulosa cells were harvested and incubated in triplicate for 24 h in M199 with or without ovine FSH or ovine LH. Plasma and culture media samples were assayed for inhibin, estradiol (E2), androstenedione (A4), and testosterone (T) by specific RIA. After correcting for hematocrit, ovarian secretion rates were calculated from the product of the plasma concentration and flow rate. The rate of ovarian inhibin secretion during the luteal phase was similar from ovaries categorized on the basis of presence of luteal tissue (1.0 ± 0.3 and 0.9 ± 0.5 ng/min for CL present and absent, respectively), confirming that the ovine CL does not secrete appreciable amounts of inhibin. Inhibin secretion was higher (p < 0.05) at 12 h after PG-induced luteolysis but not at 24 or 48 h compared to values for luteal phase control ewes. Although ovaries containing large estrogenic follicles (≥ 4 mm in diameter and classified as estrogenic from in vitro criteria) secreted the most inhibin (55%; p < 0.05), both ovaries containing large nonestrogenic follicles (33%) and small (11%; less than 4 mm in diameter) follicles secreted appreciable amounts of inhibin. This contrasted strongly with E2 where greater than 80% of the steroid was secreted by large estrogenic follicles. The rate of ovarian inhibin secretion was positively correlated (p < 0.05) with the rate of E2, A4, and T secretion. Overall, there was no significant effect of stage of cycle on follicular inhibin content after 2 h incubation in vitro, release of inhibin by follicles incubated in vitro, or synthesis of inhibin by granulosa cells cultured in vitro. FSH and LH had no effect on the production of either inhibin or estradiol by cultured granulosa cells. Follicular diameter was positively correlated (p < 0.001) with follicular inhibin and steroid release. Follicular inhibin content after 2 h incubation in vitro was more highly correlated with inhibin release by incubated follicles (r = 0.7; p < 0.001) than with inhibin synthesis by granulosa cells in vitro (0.4; p < 0.01). Follicular inhibin content after 2 h incubation in vitro was lower (p < 0.01) in small follicles but was unaffected by the estrogenicity of large follicles. Large estrogenic follicles incubated in vitro released more (p < 0.01) inhibin than large nonestrogenic follicles, which in turn released more inhibin (p < 0.01) than small follicles. In contrast, inhibin production by granulosa cells, on a per cell basis, was greatly elevated (p < 0.001) from cells isolated from large estrogenic follicles, but not from large nonestrogenic or small follicles. This indicates the existence of a population of large nonestrogenic follicles that releases appreciable quantities of inhibin, but does not necessarily reflect the capacity of those follicles’ granulosa cells to synthesize inhibin. The ovarian secretion rate of inhibin, however, was more highly correlated with follicular inhibin content after 2 h incubation in vitro (r = 0.6; p < 0.001) than either follicular (r = 0.5; p < 0.01) or granulosa cell (r = 0.4; p < 0.01) inhibin production in vitro. We conclude that inhibin, like estradiol, is secreted by large estrogenic follicles but that inhibin, unlike estradiol, is also secreted in appreciable quantities by large nonestrogenic follicles and small follicles. This range of follicular sources and the insensitivity of their granulosa cells to gonadotrophic stimulation may explain the lack of variation in inhibits secretion at different stages of the estrous cycle." @default.
- W2162628872 created "2016-06-24" @default.
- W2162628872 creator A5022230895 @default.
- W2162628872 creator A5039781020 @default.
- W2162628872 creator A5045816546 @default.
- W2162628872 creator A5080079870 @default.
- W2162628872 date "1991-03-01" @default.
- W2162628872 modified "2023-10-16" @default.
- W2162628872 title "The Effect of Stage of Estrous Cycle and Follicular Maturation on Ovarian Inhibin Production in Sheep1" @default.
- W2162628872 cites W192409273 @default.
- W2162628872 cites W1966857123 @default.
- W2162628872 cites W1974950175 @default.
- W2162628872 cites W1976529899 @default.
- W2162628872 cites W1989946455 @default.
- W2162628872 cites W1995827981 @default.
- W2162628872 cites W1996054293 @default.
- W2162628872 cites W2000915587 @default.
- W2162628872 cites W2002827657 @default.
- W2162628872 cites W2015045119 @default.
- W2162628872 cites W2017050470 @default.
- W2162628872 cites W2017712599 @default.
- W2162628872 cites W2021666654 @default.
- W2162628872 cites W2024797729 @default.
- W2162628872 cites W2026672898 @default.
- W2162628872 cites W2028327235 @default.
- W2162628872 cites W2035576731 @default.
- W2162628872 cites W2037039077 @default.
- W2162628872 cites W2037261958 @default.
- W2162628872 cites W2038843802 @default.
- W2162628872 cites W2039913810 @default.
- W2162628872 cites W2044421863 @default.
- W2162628872 cites W2049198626 @default.
- W2162628872 cites W2053773876 @default.
- W2162628872 cites W2067041674 @default.
- W2162628872 cites W2071828339 @default.
- W2162628872 cites W2076644768 @default.
- W2162628872 cites W2094614243 @default.
- W2162628872 cites W2097387517 @default.
- W2162628872 cites W2098117708 @default.
- W2162628872 cites W2103270298 @default.
- W2162628872 cites W2104390259 @default.
- W2162628872 cites W2105330401 @default.
- W2162628872 cites W2121541527 @default.
- W2162628872 cites W2123625185 @default.
- W2162628872 cites W2131709519 @default.
- W2162628872 cites W2136140872 @default.
- W2162628872 cites W2139448460 @default.
- W2162628872 cites W2149186376 @default.
- W2162628872 cites W2149765570 @default.
- W2162628872 cites W2768272984 @default.
- W2162628872 doi "https://doi.org/10.1095/biolreprod44.3.483" @default.
- W2162628872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2015365" @default.
- W2162628872 hasPublicationYear "1991" @default.
- W2162628872 type Work @default.
- W2162628872 sameAs 2162628872 @default.
- W2162628872 citedByCount "37" @default.
- W2162628872 countsByYear W21626288722012 @default.
- W2162628872 countsByYear W21626288722013 @default.
- W2162628872 countsByYear W21626288722014 @default.
- W2162628872 countsByYear W21626288722016 @default.
- W2162628872 countsByYear W21626288722018 @default.
- W2162628872 countsByYear W21626288722023 @default.
- W2162628872 crossrefType "journal-article" @default.
- W2162628872 hasAuthorship W2162628872A5022230895 @default.
- W2162628872 hasAuthorship W2162628872A5039781020 @default.
- W2162628872 hasAuthorship W2162628872A5045816546 @default.
- W2162628872 hasAuthorship W2162628872A5080079870 @default.
- W2162628872 hasBestOaLocation W21626288721 @default.
- W2162628872 hasConcept C126322002 @default.
- W2162628872 hasConcept C134018914 @default.
- W2162628872 hasConcept C143228043 @default.
- W2162628872 hasConcept C16685009 @default.
- W2162628872 hasConcept C187785154 @default.
- W2162628872 hasConcept C1998276 @default.
- W2162628872 hasConcept C2776608385 @default.
- W2162628872 hasConcept C2776683971 @default.
- W2162628872 hasConcept C2777330693 @default.
- W2162628872 hasConcept C2777702977 @default.
- W2162628872 hasConcept C2777911890 @default.
- W2162628872 hasConcept C2778324911 @default.
- W2162628872 hasConcept C2780536345 @default.
- W2162628872 hasConcept C2780664492 @default.
- W2162628872 hasConcept C71315377 @default.
- W2162628872 hasConcept C71924100 @default.
- W2162628872 hasConcept C86803240 @default.
- W2162628872 hasConceptScore W2162628872C126322002 @default.
- W2162628872 hasConceptScore W2162628872C134018914 @default.
- W2162628872 hasConceptScore W2162628872C143228043 @default.
- W2162628872 hasConceptScore W2162628872C16685009 @default.
- W2162628872 hasConceptScore W2162628872C187785154 @default.
- W2162628872 hasConceptScore W2162628872C1998276 @default.
- W2162628872 hasConceptScore W2162628872C2776608385 @default.
- W2162628872 hasConceptScore W2162628872C2776683971 @default.
- W2162628872 hasConceptScore W2162628872C2777330693 @default.
- W2162628872 hasConceptScore W2162628872C2777702977 @default.
- W2162628872 hasConceptScore W2162628872C2777911890 @default.
- W2162628872 hasConceptScore W2162628872C2778324911 @default.
- W2162628872 hasConceptScore W2162628872C2780536345 @default.