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• W2162788765 abstract "Catalyzed reporter deposition (CARD) is a technique that allows amplification of routine immunolabeling in light microscopy. This procedure takes advantage of the horseradish peroxidase (HRP) from an HRP-avidin complex to catalyze the accumulation of reporter-conjugated tyramine (a phenolic compound) onto a surface displaying biotinylated antigen-antibody complexes. The large amount of labeled tyramine deposited allows the detection of an antigenic site with multiple reporter molecules. In this study we modified this amplification protocol to combine it with the immunogold technique for the ultrastructural localization of antigens in electron microscopy. We constructed various tyramide conjugates that permit the combination of this amplification method with a particulate colloidal gold marker. The new probes yield results of high specificity and enhanced intensity. Assessment of the level of resolution of the labeling has demonstrated that, in spite of the amplification, the resolution remains very good. Therefore, once associated, the immunogold and the CARD techniques lead to specific, high-resolution, sensitive and amplified signals that exhibit the advantages of both approaches." @default.
• W2162788765 created "2016-06-24" @default.
• W2162788765 creator A5085781030 @default.
• W2162788765 creator A5089576069 @default.
• W2162788765 date "1999-04-01" @default.
• W2162788765 modified "2023-10-14" @default.
• W2162788765 title "Immunogold Signal Amplification: Application of the CARD Approach to Electron Microscopy" @default.
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• W2162788765 doi "https://doi.org/10.1177/002215549904700401" @default.
• W2162788765 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10082744" @default.
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