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- W2162912192 abstract "In a validation trial of a DNA microarray test for chlamydiae we repeatedly observed false-positive PCR amplicons from truly negative samples and non-template controls. Various PCR tests, microarray hybridization and DNA sequencing, revealed that residual Escherichia coli DNA from thermostable DNA polymerases was the cause of this cross-reaction. A subsequent survey showed that only five out of 23 commercial polymerases were free of E. coli DNA. When designing generic oligonucleotide sequences for PCR and PCR microarray-based assays one should be aware of such possible internal contamination, particularly when the target organism is E. coli." @default.
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- W2162912192 date "2007-04-01" @default.
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- W2162912192 title "Residual DNA in thermostable DNA polymerases – a cause of irritation in diagnostic PCR and microarray assays" @default.
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- W2162912192 doi "https://doi.org/10.1016/j.biologicals.2006.04.001" @default.
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