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- W2162946495 abstract "Introduction Within the past decade, shotgun sequencing has been demonstrated to be a powerful tool in sequencing the genomes of several organisms (1). Due to limitations of only several hundred consecutive bases with adequate resolution, techniques needed to be developed to piece together billions of bases into consecutive order. There are several emerging technologies that make sequencing faster and generate short sequences. 454 Life SciencesTM utilizes the pyrosequencing technique and generates sequences that are about 100 bases in length (2), Illumina®’s sequencing-by-synthesis method yields read lengths of about 25 base pairs (3), and the sequencing-by-ligation technique yields fragments about 20 base pairs in length (4). The genomes from many organisms including Drosophila melanogaster, Escherichia coli, mouse and of course human have been fully sequenced and assembled and are available in multiple databases for use by the public. However, many researchers are not working with sequences that have been assembled and published." @default.
- W2162946495 created "2016-06-24" @default.
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- W2162946495 date "2007-01-01" @default.
- W2162946495 modified "2023-09-24" @default.
- W2162946495 title "Sequence Assembly and Mutation Detection with Mutation Surveyor" @default.
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