Matches in SemOpenAlex for { <https://semopenalex.org/work/W2163013044> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2163013044 abstract "Objective To construct and identify the recombinant adenovirus containing SOCS3, detect SOCS3 expression level in HEK293 cells and provide valuable reference for gene therapy of myocarditis. Methods The SOCS3 gene was cloned by RT-PCR after total RNA extracted from peritoneal macrophage of rat, then subcloned with shuttle plasmid pAdtrack-CMV containing GFP reporter gene. PAdeasy-1 adenovirus system was used for co-transfecting shuttle plasmid and frame plasmid to Bj5183 cells. Reconstructed adenovirus was transfected to HEK293 cell after linearisation and mRNA and protein level of SOCS3 in the cell were detected. Results SOCS3 gene was cloned and recombinant adenovirus pAd-SOCS3-GFP was constructed successfully through pAdeasy-1 system. After linearisation, recombinant adenovirus was transfected to HEK293 cell and the mRNA and protein level of SOCS3 in the cell were both significantly increased. Conclusion Recombinant SOCS3 adenovirus has been constructed successfully and could be used for transfection in vivo or in vitro to treat inflammatory disease, for example, myocarditis." @default.
- W2163013044 created "2016-06-24" @default.
- W2163013044 creator A5003133092 @default.
- W2163013044 creator A5042958124 @default.
- W2163013044 creator A5052169513 @default.
- W2163013044 creator A5076520427 @default.
- W2163013044 creator A5078108644 @default.
- W2163013044 date "2011-10-01" @default.
- W2163013044 modified "2023-09-24" @default.
- W2163013044 title "Construction and identification adenovirus of SOCS3" @default.
- W2163013044 doi "https://doi.org/10.1136/heartjnl-2011-300867.148" @default.
- W2163013044 hasPublicationYear "2011" @default.
- W2163013044 type Work @default.
- W2163013044 sameAs 2163013044 @default.
- W2163013044 citedByCount "0" @default.
- W2163013044 crossrefType "journal-article" @default.
- W2163013044 hasAuthorship W2163013044A5003133092 @default.
- W2163013044 hasAuthorship W2163013044A5042958124 @default.
- W2163013044 hasAuthorship W2163013044A5052169513 @default.
- W2163013044 hasAuthorship W2163013044A5076520427 @default.
- W2163013044 hasAuthorship W2163013044A5078108644 @default.
- W2163013044 hasBestOaLocation W21630130441 @default.
- W2163013044 hasConcept C104317684 @default.
- W2163013044 hasConcept C111599444 @default.
- W2163013044 hasConcept C153911025 @default.
- W2163013044 hasConcept C159047783 @default.
- W2163013044 hasConcept C174510640 @default.
- W2163013044 hasConcept C22744801 @default.
- W2163013044 hasConcept C40767141 @default.
- W2163013044 hasConcept C54009773 @default.
- W2163013044 hasConcept C54355233 @default.
- W2163013044 hasConcept C71924100 @default.
- W2163013044 hasConcept C86803240 @default.
- W2163013044 hasConceptScore W2163013044C104317684 @default.
- W2163013044 hasConceptScore W2163013044C111599444 @default.
- W2163013044 hasConceptScore W2163013044C153911025 @default.
- W2163013044 hasConceptScore W2163013044C159047783 @default.
- W2163013044 hasConceptScore W2163013044C174510640 @default.
- W2163013044 hasConceptScore W2163013044C22744801 @default.
- W2163013044 hasConceptScore W2163013044C40767141 @default.
- W2163013044 hasConceptScore W2163013044C54009773 @default.
- W2163013044 hasConceptScore W2163013044C54355233 @default.
- W2163013044 hasConceptScore W2163013044C71924100 @default.
- W2163013044 hasConceptScore W2163013044C86803240 @default.
- W2163013044 hasLocation W21630130441 @default.
- W2163013044 hasOpenAccess W2163013044 @default.
- W2163013044 hasPrimaryLocation W21630130441 @default.
- W2163013044 hasRelatedWork W2348551073 @default.
- W2163013044 hasRelatedWork W2351954607 @default.
- W2163013044 hasRelatedWork W2353838075 @default.
- W2163013044 hasRelatedWork W2354296025 @default.
- W2163013044 hasRelatedWork W2356563149 @default.
- W2163013044 hasRelatedWork W2356625414 @default.
- W2163013044 hasRelatedWork W2357649990 @default.
- W2163013044 hasRelatedWork W2359841857 @default.
- W2163013044 hasRelatedWork W2361821711 @default.
- W2163013044 hasRelatedWork W2373189896 @default.
- W2163013044 hasRelatedWork W2376184979 @default.
- W2163013044 hasRelatedWork W2377849370 @default.
- W2163013044 hasRelatedWork W2378428495 @default.
- W2163013044 hasRelatedWork W2380192975 @default.
- W2163013044 hasRelatedWork W2380731165 @default.
- W2163013044 hasRelatedWork W2381101320 @default.
- W2163013044 hasRelatedWork W2388304400 @default.
- W2163013044 hasRelatedWork W2389680535 @default.
- W2163013044 hasRelatedWork W2394426760 @default.
- W2163013044 hasRelatedWork W2412387176 @default.
- W2163013044 isParatext "false" @default.
- W2163013044 isRetracted "false" @default.
- W2163013044 magId "2163013044" @default.
- W2163013044 workType "article" @default.