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- W216302928 abstract "Abstract Medium conditioned by human placental tissue was found to stimulate granulocytic and monocytic colony formation by human marrow cells in semisolid agar cultures. The colony-stimulating activity of unfractionated conditioned medium was equivalent to the activity of standard peripheral blood underlayers. Placentas were a reliable source of active material, and one placenta provided enough material to stimulate 5,000–10,000 cultures of normal or leukemic cells. The colony- stimulating factor in human placental conditioned medium (CSFHPCM) was concentrated and purified 1800-fold using ammonium sulfate precipitation, calcium phosphate gel absorption, DEAE-cellulose batch absorption, gel filtration on Sephadex G-150, and polyarcylamide gel gel electrophoresis. The active factor behaved on gel filtration as a macromolecule with an apparent molecular weight of 30,000 daltons. The active factor in placental conditioned medium was not dependent on the presence of adherent marrow cells with endogenous colony-stimulating activity." @default.
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- W216302928 date "1977-04-01" @default.
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- W216302928 title "Stimulation by human placental conditioned medium of hemopoietic colony formation by human marrow cells" @default.
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- W216302928 doi "https://doi.org/10.1182/blood.v49.4.573.573" @default.
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