Matches in SemOpenAlex for { <https://semopenalex.org/work/W2163091976> ?p ?o ?g. }
- W2163091976 endingPage "746" @default.
- W2163091976 startingPage "737" @default.
- W2163091976 abstract "Abstract Genetic transformation in insects holds great promise as a tool for genetic manipulation in species of particular scientific, economic, or medical interest. A number of transposable elements have been tested recently as potential vectors for transformation in a range of insects. Minos is one of the most promising elements because it appears to be active in diverse species and has the capacity to carry large inserts. We report here the use of the Minos element as a transformation vector in the red flour beetle Tribolium castaneum (Coleoptera), an important species for comparative developmental and pest management studies. Transgenic G1 beetles were recovered from 32.4% of fertile G0's injected with a plasmid carrying a 3xP3-EGFP-marked transposon and in vitro synthesized mRNA encoding the Minos transposase. This transformation efficiency is 2.8-fold higher than that observed when using a plasmid helper. Molecular and genetic analyses show that several independent insertions can be recovered from a single injected parent, but that the majority of transformed individuals carry single Minos insertions. These results establish Minos as one of the most efficient vectors for genetic transformation in insects. In combination with piggyBac-based transgenesis, our work allows the introduction of sophisticated multicomponent genetic tools in Tribolium." @default.
- W2163091976 created "2016-06-24" @default.
- W2163091976 creator A5002824464 @default.
- W2163091976 creator A5005334960 @default.
- W2163091976 creator A5017359946 @default.
- W2163091976 creator A5053036630 @default.
- W2163091976 date "2004-06-01" @default.
- W2163091976 modified "2023-09-27" @default.
- W2163091976 title "Efficient Transformation of the Beetle Tribolium castaneum Using the Minos Transposable Element" @default.
- W2163091976 cites W1534179616 @default.
- W2163091976 cites W1939841200 @default.
- W2163091976 cites W1941119369 @default.
- W2163091976 cites W1966080460 @default.
- W2163091976 cites W1969285824 @default.
- W2163091976 cites W1973403924 @default.
- W2163091976 cites W1975104536 @default.
- W2163091976 cites W1977567457 @default.
- W2163091976 cites W1977743697 @default.
- W2163091976 cites W1979128283 @default.
- W2163091976 cites W1989322815 @default.
- W2163091976 cites W1994528729 @default.
- W2163091976 cites W1998974970 @default.
- W2163091976 cites W2002250380 @default.
- W2163091976 cites W2010970917 @default.
- W2163091976 cites W2012674191 @default.
- W2163091976 cites W2015078061 @default.
- W2163091976 cites W2017377227 @default.
- W2163091976 cites W2018442148 @default.
- W2163091976 cites W2024129308 @default.
- W2163091976 cites W2026952154 @default.
- W2163091976 cites W2028441326 @default.
- W2163091976 cites W2029880048 @default.
- W2163091976 cites W2034274710 @default.
- W2163091976 cites W2045009423 @default.
- W2163091976 cites W2056900382 @default.
- W2163091976 cites W2057333689 @default.
- W2163091976 cites W2063728571 @default.
- W2163091976 cites W2071752035 @default.
- W2163091976 cites W2076345037 @default.
- W2163091976 cites W2084357002 @default.
- W2163091976 cites W2084445314 @default.
- W2163091976 cites W2086689509 @default.
- W2163091976 cites W2089430842 @default.
- W2163091976 cites W2103960876 @default.
- W2163091976 cites W2106936924 @default.
- W2163091976 cites W2110643873 @default.
- W2163091976 cites W2111478668 @default.
- W2163091976 cites W2117104012 @default.
- W2163091976 cites W2127068865 @default.
- W2163091976 cites W2140538490 @default.
- W2163091976 cites W2155970893 @default.
- W2163091976 cites W2159334577 @default.
- W2163091976 cites W2164339920 @default.
- W2163091976 cites W2169250438 @default.
- W2163091976 cites W2169831229 @default.
- W2163091976 cites W2770975681 @default.
- W2163091976 doi "https://doi.org/10.1534/genetics.103.023085" @default.
- W2163091976 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1470898" @default.
- W2163091976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15238525" @default.
- W2163091976 hasPublicationYear "2004" @default.
- W2163091976 type Work @default.
- W2163091976 sameAs 2163091976 @default.
- W2163091976 citedByCount "74" @default.
- W2163091976 countsByYear W21630919762012 @default.
- W2163091976 countsByYear W21630919762013 @default.
- W2163091976 countsByYear W21630919762014 @default.
- W2163091976 countsByYear W21630919762015 @default.
- W2163091976 countsByYear W21630919762016 @default.
- W2163091976 countsByYear W21630919762017 @default.
- W2163091976 countsByYear W21630919762018 @default.
- W2163091976 countsByYear W21630919762019 @default.
- W2163091976 countsByYear W21630919762020 @default.
- W2163091976 countsByYear W21630919762021 @default.
- W2163091976 countsByYear W21630919762022 @default.
- W2163091976 crossrefType "journal-article" @default.
- W2163091976 hasAuthorship W2163091976A5002824464 @default.
- W2163091976 hasAuthorship W2163091976A5005334960 @default.
- W2163091976 hasAuthorship W2163091976A5017359946 @default.
- W2163091976 hasAuthorship W2163091976A5053036630 @default.
- W2163091976 hasBestOaLocation W21630919761 @default.
- W2163091976 hasConcept C104317684 @default.
- W2163091976 hasConcept C107966497 @default.
- W2163091976 hasConcept C121332964 @default.
- W2163091976 hasConcept C141231307 @default.
- W2163091976 hasConcept C172510086 @default.
- W2163091976 hasConcept C185544564 @default.
- W2163091976 hasConcept C186453547 @default.
- W2163091976 hasConcept C204241405 @default.
- W2163091976 hasConcept C22508944 @default.
- W2163091976 hasConcept C22744801 @default.
- W2163091976 hasConcept C2780314645 @default.
- W2163091976 hasConcept C4918238 @default.
- W2163091976 hasConcept C519853106 @default.
- W2163091976 hasConcept C54355233 @default.
- W2163091976 hasConcept C59822182 @default.
- W2163091976 hasConcept C73247094 @default.
- W2163091976 hasConcept C86803240 @default.
- W2163091976 hasConcept C87073359 @default.