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- W2163165094 abstract "Abstrak. Penelitian ini bertujuan untuk melakukan screening bakteri proteolitik termofilik dari sumber air panas Singgahan Tuban. Isolasi dilakukan menggunakan media Luria Bertany dan screening dilakukan menggunakan media agar skim milk 6%. Indeks Proteolitik (IP) bakteri ditentukan dengan membandingkan diameter zona bening dan diameter zona pertumbuhan koloni. IP yang relatif besar dipilih untuk diproduksi dan ditentukan aktivitas proteolitiknya menggunakan media yang mengandung kasein 2%. Pengukuran aktivitas enzim protease menggunakan reagen Follin Ciocalteu, yang diukur dengan spektrofotometer UV-Vis pada panjang gelombang 750 nm. Hasil screening menunjukkan bahwa dari 76 isolat bakteri yang diperoleh, 28 isolat diantaranya mampu menghasilkan protease. Isolat dengan kode ST-57 memiliki IP dan aktivitas terbesar yaitu 7,0, dan 0,2489 Unit/mL. Kata kunci: Bakteri proteolitik termofilik, indeks proteolitik, aktivitas enzim protease Abstract. This research aimed to screening proteolytic thermophilic bacteria from hot springs Singgahan Tuban. Isolation was done Luria Bertany medium and screening was done by using 6% skim milk agar medium. Proteolytic index (IP) bacteria determined by comparing the diameter of clear zone and colony growth diameter of the growth zone. The big IP was choosen for being produce and to determine the proteolytic activity using casein 2 % as a medium. Enzymes from proteolytic bacteria isolate then produced and measured it proteolytic activity used medium containing 2% casein. Enzyme activity test used Follin Ciocalteu reagent, measured with UV-Vis spectrophotometer at 750 nm. Screening yield from 76 isolates, 28 isolates able to produce proteases. Isolates with code ST-57 had an IP and the greatest activity 7.0, and 0.2489 Units/mL. Keywords: Proteolytic thermophilic bacteria, proteolytic index, protease enzyme activity" @default.
- W2163165094 created "2016-06-24" @default.
- W2163165094 creator A5038763643 @default.
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- W2163165094 date "2014-10-12" @default.
- W2163165094 modified "2023-09-24" @default.
- W2163165094 title "SCREENING BAKTERI PROTEOLITIK TERMOFILIK DARI SUMBER AIR PANAS SINGGAHAN TUBAN SCREENING PROTEOLYTIC THERMOPHILIC BACTERIA FROM HOT SPRINGS SINGGAHAN TUBAN" @default.
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