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• W2163202857 abstract "The chicken skeletal alpha-actin gene promoter region provides at least a 75-fold-greater transcriptional activity in muscle cells than in fibroblasts. The cis-acting sequences required for cell type-restricted expression within this 200-base-pair (bp) region were elucidated by chloramphenicol acetyltransferase assays of site-directed Bg/II linker-scanning mutations transiently transfected into primary cultures. Four positive cis-acting elements were identified and are required for efficient transcriptional activity in myogenic cells. These elements, conserved across vertebrate evolution, include the ATAAAA box (-24 bp), paired CCAAT-box-associated repeats (CBARs; at -83 bp and -127 bp), and the upstream T+A-rich regulatory sequence (at -176 bp). Basal transcriptional activity in fibroblasts was not as dependent on the upstream CBAR or regions of the upstream T+A-rich regulatory sequence. Transfection experiments provided evidence that positive regulatory factors required for alpha-actin expression in fibroblasts are limiting. In addition, negative cis-acting elements were detected and found closely associated with the G+C-rich sequences that surround the paired CBARs. Negative elements may have a role in restricting developmentally timed expression in myoblasts and appear to inhibit promoter activity in nonmyogenic cells. Cell type-specific expression of the skeletal alpha-actin gene promoter is regulated by combinatorial and possibly competitive interactions between multiple positive and negative cis-acting elements." @default.
• W2163202857 created "2016-06-24" @default.
• W2163202857 creator A5038896281 @default.
• W2163202857 creator A5070434265 @default.
• W2163202857 date "1990-02-01" @default.
• W2163202857 modified "2023-09-27" @default.
• W2163202857 title "A combination of closely associated positive and negative cis-acting promoter elements regulates transcription of the skeletal alpha-actin gene." @default.
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• W2163202857 doi "https://doi.org/10.1128/mcb.10.2.528" @default.
• W2163202857 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/360830" @default.
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