Matches in SemOpenAlex for { <https://semopenalex.org/work/W2163318454> ?p ?o ?g. }
- W2163318454 endingPage "346" @default.
- W2163318454 startingPage "339" @default.
- W2163318454 abstract "Tomoregulin-1, a type-I transmembrane protein with two follistatin modules, a unique epidermal growth factor (EGF) domain and a short, highly conserved cytoplasmic tail, was studied. A number of hematopoietic cell lines (L1210, CEM, Jurkat, U937, K562, JY, THP-1 and T2) express tomoregulin-1 endogenously. In these cells, apoptosis was induced by an antiserum (C29) and purified IgG against the follistatin modules, but not by antisera against the EGF- domain or the cytoplasmic tail. Furthermore, C29 induced apoptosis in tomoregulin-1-, but not in mock-transfected cells. Apoptosis was monitored through genomic DNA fragmentation, annexin-V staining and caspase-3 activation. Treatment of the cells with C29 in the presence of H89 (a Ser/Thr kinase inhibitor) or 8'-bromo-cyclicAMP revealed that apoptosis was mediated by a cAMP-dependent Ser/Thr kinase. Moreover, C29 increased (cAMP)i over 5-fold. Together, these data suggest that the C29 antiserum against tomoregulin-1 induces apoptosis of hematopoietic cells. During the application of a differential screening approach to Xenopus laevis pituitary, several novel cDNAs have been discovered (1). One of these cDNAs was designated 7365 and encodes a protein structurally related to the transmembrane precursors of the epidermal/transforming growth factor (EGF/TGF-·) family with a unique EGF domain, in that all characteristics are present except for an essential Arg residue (2). In addition, the 7365 protein contains a signal peptide, 2 extracellular follistatin (FS) domains, a transmembrane domain and a short cytoplasmic C-terminal region. Two papers described expression patterns of 7365 (3, 4). The mouse and human orthologues (GenBank, U19878) were cloned, and the name tomoregulin-1 was proposed for this protein (3). A striking degree of amino acid sequence identity between mammalian and amphibian tomoregulin-1 was found, in particular in the 29 amino acid long C-terminal portion of the protein (4). The short cytoplasmic tail is nearly identical (97%) in mammals and Xenopus laevis. In view of the above characteristics, we decided to investigate the role of tomoregulin-1 in the proliferation and apoptosis of mammalian cell lines. For this study, antisera directed against different parts of tomoregulin-1 were employed. The use of antisera to unravel signaling pathways has been successful for CD95 and the amyloid precursor protein (5, 6). Following the two reports of expression patterns (3, 4), evidence that tomoregulin-1 is involved in Ser/Thr kinase-dependent apoptosis of hematopoietic cells is provided here." @default.
- W2163318454 created "2016-06-24" @default.
- W2163318454 creator A5005257877 @default.
- W2163318454 creator A5030923034 @default.
- W2163318454 creator A5034641400 @default.
- W2163318454 creator A5055405631 @default.
- W2163318454 creator A5074097963 @default.
- W2163318454 creator A5074836954 @default.
- W2163318454 date "2006-01-01" @default.
- W2163318454 modified "2023-09-27" @default.
- W2163318454 title "Induction of apoptosis in hematopoietic cells with an antibody against tomoregulin-1." @default.
- W2163318454 cites W1586205496 @default.
- W2163318454 cites W1600128976 @default.
- W2163318454 cites W1642763135 @default.
- W2163318454 cites W1965834253 @default.
- W2163318454 cites W1966527292 @default.
- W2163318454 cites W1966967877 @default.
- W2163318454 cites W1968685674 @default.
- W2163318454 cites W1982021183 @default.
- W2163318454 cites W1984445750 @default.
- W2163318454 cites W2008652385 @default.
- W2163318454 cites W2009234259 @default.
- W2163318454 cites W2010298858 @default.
- W2163318454 cites W2020314225 @default.
- W2163318454 cites W2021126994 @default.
- W2163318454 cites W2026205355 @default.
- W2163318454 cites W2031399745 @default.
- W2163318454 cites W2033494109 @default.
- W2163318454 cites W2040760345 @default.
- W2163318454 cites W2042031605 @default.
- W2163318454 cites W2059231375 @default.
- W2163318454 cites W2068261927 @default.
- W2163318454 cites W2076629304 @default.
- W2163318454 cites W2076672225 @default.
- W2163318454 cites W2080510044 @default.
- W2163318454 cites W2084436635 @default.
- W2163318454 cites W2086402875 @default.
- W2163318454 cites W2088758828 @default.
- W2163318454 cites W2093865116 @default.
- W2163318454 cites W2097664380 @default.
- W2163318454 cites W2100314556 @default.
- W2163318454 cites W2122209411 @default.
- W2163318454 cites W2125771413 @default.
- W2163318454 cites W2128108117 @default.
- W2163318454 cites W2131444791 @default.
- W2163318454 cites W2140767277 @default.
- W2163318454 cites W2152748860 @default.
- W2163318454 cites W2157658806 @default.
- W2163318454 cites W2165469800 @default.
- W2163318454 cites W2167779491 @default.
- W2163318454 cites W2312283262 @default.
- W2163318454 cites W2333249240 @default.
- W2163318454 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16475715" @default.
- W2163318454 hasPublicationYear "2006" @default.
- W2163318454 type Work @default.
- W2163318454 sameAs 2163318454 @default.
- W2163318454 citedByCount "2" @default.
- W2163318454 countsByYear W21633184542012 @default.
- W2163318454 crossrefType "journal-article" @default.
- W2163318454 hasAuthorship W2163318454A5005257877 @default.
- W2163318454 hasAuthorship W2163318454A5030923034 @default.
- W2163318454 hasAuthorship W2163318454A5034641400 @default.
- W2163318454 hasAuthorship W2163318454A5055405631 @default.
- W2163318454 hasAuthorship W2163318454A5074097963 @default.
- W2163318454 hasAuthorship W2163318454A5074836954 @default.
- W2163318454 hasConcept C153911025 @default.
- W2163318454 hasConcept C170301204 @default.
- W2163318454 hasConcept C190062978 @default.
- W2163318454 hasConcept C190283241 @default.
- W2163318454 hasConcept C2776362946 @default.
- W2163318454 hasConcept C31573885 @default.
- W2163318454 hasConcept C43759708 @default.
- W2163318454 hasConcept C54009773 @default.
- W2163318454 hasConcept C54355233 @default.
- W2163318454 hasConcept C55493867 @default.
- W2163318454 hasConcept C81885089 @default.
- W2163318454 hasConcept C86803240 @default.
- W2163318454 hasConcept C95444343 @default.
- W2163318454 hasConcept C98424977 @default.
- W2163318454 hasConceptScore W2163318454C153911025 @default.
- W2163318454 hasConceptScore W2163318454C170301204 @default.
- W2163318454 hasConceptScore W2163318454C190062978 @default.
- W2163318454 hasConceptScore W2163318454C190283241 @default.
- W2163318454 hasConceptScore W2163318454C2776362946 @default.
- W2163318454 hasConceptScore W2163318454C31573885 @default.
- W2163318454 hasConceptScore W2163318454C43759708 @default.
- W2163318454 hasConceptScore W2163318454C54009773 @default.
- W2163318454 hasConceptScore W2163318454C54355233 @default.
- W2163318454 hasConceptScore W2163318454C55493867 @default.
- W2163318454 hasConceptScore W2163318454C81885089 @default.
- W2163318454 hasConceptScore W2163318454C86803240 @default.
- W2163318454 hasConceptScore W2163318454C95444343 @default.
- W2163318454 hasConceptScore W2163318454C98424977 @default.
- W2163318454 hasLocation W21633184541 @default.
- W2163318454 hasLocation W21633184542 @default.
- W2163318454 hasOpenAccess W2163318454 @default.
- W2163318454 hasPrimaryLocation W21633184541 @default.
- W2163318454 hasRelatedWork W1539625195 @default.