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- W2163347296 abstract "Abstract To evaluate the synthesis of acetaldehyde from threonine during growth of yogurt cultures, Streptococcus thermophilus MS1 and Lactobacillus bulgaricus MR1 were grown in defined medium in which 10% of the total threonine was composed of L-[carbon-14(U)] threonine. Acetaldehyde production was monitored by formation of 2,4-dinitrophenylhydrazone followed by separation and analysis using high performance liquid chromatography. After growth for 8h at 42°C, approximately 2.0% of the total acetaldehyde (780.4 nmol) produced was from L-[carbon-14]threonine. Threonine aldolase activity was determined in cell-free extracts from S. thermophilus and L. bulgaricus grown in Elliker broth. Increasing incubation temperature from 30 to 42°C decreased threonine aldolase activity in cells of the streptococcus harvested after 8h of incubation. Effect of incubation temperature was more dramatic in cells harvested after 18h where the activity of cells grown at 42°C was 89% lower than that of cells grown at 30°C. Cell extracts from S. thermophilus MS1 possessed higher threonine aldolase activity than did those from L. bulgaricus MR1. Increased assay temperature from 30 to 42°C increased threonine aldolase activity in S. thermophilus MS1." @default.
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- W2163347296 date "1986-05-01" @default.
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- W2163347296 title "Evaluating Acetaldehyde Synthesis from L-[14C(U)] Threonine by Streptococcus thermophilus and Lactobacillus bulgaricus" @default.
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- W2163347296 doi "https://doi.org/10.3168/jds.s0022-0302(86)80526-4" @default.
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