Matches in SemOpenAlex for { <https://semopenalex.org/work/W2163406890> ?p ?o ?g. }
- W2163406890 endingPage "4987" @default.
- W2163406890 startingPage "4977" @default.
- W2163406890 abstract "Most restriction endonucleases, including FokI, interact with two copies of their recognition sequence before cutting DNA. On DNA with two sites they act in cis looping out the intervening DNA. While many restriction enzymes operate symmetrically at palindromic sites, FokI acts asymmetrically at a non-palindromic site. The directionality of its sequence means that two FokI sites can be bridged in either parallel or anti-parallel alignments. Here we show by biochemical and single-molecule biophysical methods that FokI aligns two recognition sites on separate DNA molecules in parallel and that the parallel arrangement holds for sites in the same DNA regardless of whether they are in inverted or repeated orientations. The parallel arrangement dictates the topology of the loop trapped between sites in cis : the loop from inverted sites has a simple 180° bend, while that with repeated sites has a convoluted 360° turn. The ability of FokI to act at asymmetric sites thus enabled us to identify the synapse geometry for sites in trans and in cis , which in turn revealed the relationship between synapse geometry and loop topology." @default.
- W2163406890 created "2016-06-24" @default.
- W2163406890 creator A5002177010 @default.
- W2163406890 creator A5012182700 @default.
- W2163406890 creator A5021651233 @default.
- W2163406890 creator A5027190305 @default.
- W2163406890 creator A5045775763 @default.
- W2163406890 date "2012-02-23" @default.
- W2163406890 modified "2023-09-27" @default.
- W2163406890 title "DNA looping by FokI: the impact of synapse geometry on loop topology at varied site orientations" @default.
- W2163406890 cites W1518916247 @default.
- W2163406890 cites W1521429682 @default.
- W2163406890 cites W1947861370 @default.
- W2163406890 cites W1968806285 @default.
- W2163406890 cites W1969287652 @default.
- W2163406890 cites W1977113549 @default.
- W2163406890 cites W1977445167 @default.
- W2163406890 cites W1978870223 @default.
- W2163406890 cites W1992190476 @default.
- W2163406890 cites W1996338282 @default.
- W2163406890 cites W2009697993 @default.
- W2163406890 cites W2010709173 @default.
- W2163406890 cites W2014482899 @default.
- W2163406890 cites W2041473063 @default.
- W2163406890 cites W2045455470 @default.
- W2163406890 cites W2047895017 @default.
- W2163406890 cites W2056921859 @default.
- W2163406890 cites W2057414183 @default.
- W2163406890 cites W2057556482 @default.
- W2163406890 cites W2059130766 @default.
- W2163406890 cites W2062216156 @default.
- W2163406890 cites W2063973770 @default.
- W2163406890 cites W2067453637 @default.
- W2163406890 cites W2070196801 @default.
- W2163406890 cites W2081949485 @default.
- W2163406890 cites W2083584270 @default.
- W2163406890 cites W2095611420 @default.
- W2163406890 cites W2100303812 @default.
- W2163406890 cites W2112331024 @default.
- W2163406890 cites W2114759215 @default.
- W2163406890 cites W2115526118 @default.
- W2163406890 cites W2117191068 @default.
- W2163406890 cites W2117717704 @default.
- W2163406890 cites W2118473805 @default.
- W2163406890 cites W2118514248 @default.
- W2163406890 cites W2126237980 @default.
- W2163406890 cites W2127620494 @default.
- W2163406890 cites W2133620956 @default.
- W2163406890 cites W2135917793 @default.
- W2163406890 cites W2137168241 @default.
- W2163406890 cites W2139410228 @default.
- W2163406890 cites W2143987754 @default.
- W2163406890 cites W2147971427 @default.
- W2163406890 cites W2151143554 @default.
- W2163406890 cites W2160381044 @default.
- W2163406890 cites W2167240475 @default.
- W2163406890 cites W2172157322 @default.
- W2163406890 cites W4229769694 @default.
- W2163406890 cites W4237877668 @default.
- W2163406890 cites W4254701702 @default.
- W2163406890 doi "https://doi.org/10.1093/nar/gks183" @default.
- W2163406890 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3367207" @default.
- W2163406890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22362745" @default.
- W2163406890 hasPublicationYear "2012" @default.
- W2163406890 type Work @default.
- W2163406890 sameAs 2163406890 @default.
- W2163406890 citedByCount "11" @default.
- W2163406890 countsByYear W21634068902012 @default.
- W2163406890 countsByYear W21634068902013 @default.
- W2163406890 countsByYear W21634068902014 @default.
- W2163406890 countsByYear W21634068902016 @default.
- W2163406890 countsByYear W21634068902018 @default.
- W2163406890 countsByYear W21634068902022 @default.
- W2163406890 countsByYear W21634068902023 @default.
- W2163406890 crossrefType "journal-article" @default.
- W2163406890 hasAuthorship W2163406890A5002177010 @default.
- W2163406890 hasAuthorship W2163406890A5012182700 @default.
- W2163406890 hasAuthorship W2163406890A5021651233 @default.
- W2163406890 hasAuthorship W2163406890A5027190305 @default.
- W2163406890 hasAuthorship W2163406890A5045775763 @default.
- W2163406890 hasBestOaLocation W21634068901 @default.
- W2163406890 hasConcept C100594029 @default.
- W2163406890 hasConcept C104317684 @default.
- W2163406890 hasConcept C114614502 @default.
- W2163406890 hasConcept C128319531 @default.
- W2163406890 hasConcept C141231307 @default.
- W2163406890 hasConcept C149737253 @default.
- W2163406890 hasConcept C169802333 @default.
- W2163406890 hasConcept C180754005 @default.
- W2163406890 hasConcept C184720557 @default.
- W2163406890 hasConcept C2778112365 @default.
- W2163406890 hasConcept C2781308329 @default.
- W2163406890 hasConcept C33923547 @default.
- W2163406890 hasConcept C44667518 @default.
- W2163406890 hasConcept C51603236 @default.
- W2163406890 hasConcept C54355233 @default.
- W2163406890 hasConcept C552990157 @default.
- W2163406890 hasConcept C76557 @default.