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• W2163414755 abstract "Magnetoferritin nanoparticles have been developed as high-relaxivity, functional contrast agents for MRI. Several previous techniques have relied on unloading native ferritin and re-incorporation of iron into the core, often resulting in a polydisperse sample. Here, a simplified technique is developed using commercially available horse spleen apoferritin to create monodisperse magnetoferritin. Iron oxide atoms were incorporated into the protein core via a step-wise Fe(II)Chloride addition to the protein solution under low O(2) conditions; subsequent filtration steps allow for separation of completely filled and superparamagnetic magnetoferritin from the partially filled ferritin. This method yields a monodisperse and homogenous solution of spherical particles with magnetic properties that can be used for molecular magnetic resonance imaging. With a transverse per-iron and per-particle relaxivity of 78 mM(-1) sec(-1) and 404,045 mM(-1) sec(-1), respectively, it is possible to detect ∼ 10 nM nanoparticle concentrations in vivo." @default.
• W2163414755 created "2016-06-24" @default.
• W2163414755 creator A5002902508 @default.
• W2163414755 creator A5048388860 @default.
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• W2163414755 date "2010-10-26" @default.
• W2163414755 modified "2023-10-09" @default.
• W2163414755 title "Simplified synthesis and relaxometry of magnetoferritin for magnetic resonance imaging" @default.
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• W2163414755 doi "https://doi.org/10.1002/mrm.22526" @default.
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• W2163414755 hasPublicationYear "2010" @default.
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