Matches in SemOpenAlex for { <https://semopenalex.org/work/W2163439679> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2163439679 endingPage "3465" @default.
- W2163439679 startingPage "3455" @default.
- W2163439679 abstract "ABSTRACT R2 is a non-long terminal repeat retrotransposable element that inserts itself site specifically in the 28S rRNA genes of arthropods. The 120-kDa protein encoded by R2 has been shown to cleave one strand of the 28S gene at the target site and to use the 3′ hydroxyl group generated from this nick to prime reverse transcription of its own RNA. This reaction has been termed target-primed reverse transcription (TPRT). Cleavage of the second DNA strand can occur in the presence or absence of reverse transcription but requires RNA. In this study, more sensitive in vitro assays have enabled further characterization of these reactions. R2 protein is capable of only a single round of TPRT because, once bound to the target DNA, it does not dissociate at physiological ionic strengths. Analysis of the role of RNA in the DNA cleavage reaction has revealed that the binding of RNA induces the R2 protein to form a multimeric complex. While larger complexes may form, the active component appears to be a dimer based on sedimentation studies and the change in stoichiometry of the cleavage reaction from a 1:1 ratio of protein subunit to target DNA in the absence of RNA to a 2:1 ratio of subunit to DNA target in the presence of RNA. Nonspecific RNA can also induce formation of this RNA-protein (RNP) complex, but the association of the protein with R2 RNA is stronger as revealed by its stability in 0.4 M NaCl. Finally, formation of the RNP complex gives rise to a 150-fold increase in the ability of the R2 endonuclease to find the target site. The specificity of this RNP complex is sufficiently great that it can find the 28S gene target site and conduct the TPRT reaction with total genomic DNA." @default.
- W2163439679 created "2016-06-24" @default.
- W2163439679 creator A5004758996 @default.
- W2163439679 creator A5064397076 @default.
- W2163439679 date "1998-06-01" @default.
- W2163439679 modified "2023-09-25" @default.
- W2163439679 title "RNA-Induced Changes in the Activity of the Endonuclease Encoded by the R2 Retrotransposable Element" @default.
- W2163439679 cites W128785669 @default.
- W2163439679 cites W1554644662 @default.
- W2163439679 cites W1674175723 @default.
- W2163439679 cites W1763882606 @default.
- W2163439679 cites W1769084654 @default.
- W2163439679 cites W1769159330 @default.
- W2163439679 cites W1846237601 @default.
- W2163439679 cites W1939502354 @default.
- W2163439679 cites W1970040635 @default.
- W2163439679 cites W1980680952 @default.
- W2163439679 cites W1988233506 @default.
- W2163439679 cites W1991333931 @default.
- W2163439679 cites W1995311150 @default.
- W2163439679 cites W2006183148 @default.
- W2163439679 cites W2007848061 @default.
- W2163439679 cites W2008954911 @default.
- W2163439679 cites W2014601660 @default.
- W2163439679 cites W2018226449 @default.
- W2163439679 cites W2032737565 @default.
- W2163439679 cites W2045739426 @default.
- W2163439679 cites W2053880432 @default.
- W2163439679 cites W2061054814 @default.
- W2163439679 cites W2064629259 @default.
- W2163439679 cites W2075921959 @default.
- W2163439679 cites W2085747761 @default.
- W2163439679 cites W2095346626 @default.
- W2163439679 cites W2102154371 @default.
- W2163439679 cites W2117053331 @default.
- W2163439679 cites W2128508248 @default.
- W2163439679 cites W2154703172 @default.
- W2163439679 cites W2164364144 @default.
- W2163439679 cites W2171764091 @default.
- W2163439679 doi "https://doi.org/10.1128/mcb.18.6.3455" @default.
- W2163439679 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/108926" @default.
- W2163439679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9584185" @default.
- W2163439679 hasPublicationYear "1998" @default.
- W2163439679 type Work @default.
- W2163439679 sameAs 2163439679 @default.
- W2163439679 citedByCount "34" @default.
- W2163439679 countsByYear W21634396792012 @default.
- W2163439679 countsByYear W21634396792013 @default.
- W2163439679 countsByYear W21634396792014 @default.
- W2163439679 countsByYear W21634396792015 @default.
- W2163439679 crossrefType "journal-article" @default.
- W2163439679 hasAuthorship W2163439679A5004758996 @default.
- W2163439679 hasAuthorship W2163439679A5064397076 @default.
- W2163439679 hasBestOaLocation W21634396792 @default.
- W2163439679 hasConcept C104317684 @default.
- W2163439679 hasConcept C141231307 @default.
- W2163439679 hasConcept C153911025 @default.
- W2163439679 hasConcept C2777028655 @default.
- W2163439679 hasConcept C4918238 @default.
- W2163439679 hasConcept C54355233 @default.
- W2163439679 hasConcept C552990157 @default.
- W2163439679 hasConcept C67705224 @default.
- W2163439679 hasConcept C7029365 @default.
- W2163439679 hasConcept C86803240 @default.
- W2163439679 hasConceptScore W2163439679C104317684 @default.
- W2163439679 hasConceptScore W2163439679C141231307 @default.
- W2163439679 hasConceptScore W2163439679C153911025 @default.
- W2163439679 hasConceptScore W2163439679C2777028655 @default.
- W2163439679 hasConceptScore W2163439679C4918238 @default.
- W2163439679 hasConceptScore W2163439679C54355233 @default.
- W2163439679 hasConceptScore W2163439679C552990157 @default.
- W2163439679 hasConceptScore W2163439679C67705224 @default.
- W2163439679 hasConceptScore W2163439679C7029365 @default.
- W2163439679 hasConceptScore W2163439679C86803240 @default.
- W2163439679 hasIssue "6" @default.
- W2163439679 hasLocation W21634396791 @default.
- W2163439679 hasLocation W21634396792 @default.
- W2163439679 hasLocation W21634396793 @default.
- W2163439679 hasOpenAccess W2163439679 @default.
- W2163439679 hasPrimaryLocation W21634396791 @default.
- W2163439679 hasRelatedWork W100179503 @default.
- W2163439679 hasRelatedWork W1915393084 @default.
- W2163439679 hasRelatedWork W1953861638 @default.
- W2163439679 hasRelatedWork W1990006714 @default.
- W2163439679 hasRelatedWork W1995112380 @default.
- W2163439679 hasRelatedWork W2034856837 @default.
- W2163439679 hasRelatedWork W2059195559 @default.
- W2163439679 hasRelatedWork W2098133034 @default.
- W2163439679 hasRelatedWork W2947881025 @default.
- W2163439679 hasRelatedWork W3007347855 @default.
- W2163439679 hasVolume "18" @default.
- W2163439679 isParatext "false" @default.
- W2163439679 isRetracted "false" @default.
- W2163439679 magId "2163439679" @default.
- W2163439679 workType "article" @default.