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- W2163465718 abstract "A new method has been developed for measuring the permeability coefficient, omega, of small nonelectrolytes. The method depends upon a mathematical analysis of the time course of cell volume changes in the neighborhood of the minimum volume following addition of a permeating solute to an isosmolal buffer. Coefficients determined by the minimum volume method agree with those obtained using radioactive tracers. omega for urea in human red cells was found to decrease as the volume flow, J(v), into the cell increased. Such behavior is entirely unexpected for a single uniform rate-limiting barrier on the basis of the linear phenomenological equations derived from irreversible thermodynamics. However, the present findings are consonant with a complex membrane system consisting of a tight barrier on the outer face of the human red cell membrane and a somewhat less restrictive barrier behind it closer to the inner membrane face. A theoretical analysis of such a series model has been made which makes predictions consistent with the experimental findings." @default.
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- W2163465718 date "1970-04-01" @default.
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- W2163465718 title "Determination of Urea Permeability in Red Cells by Minimum Method" @default.
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- W2163465718 doi "https://doi.org/10.1085/jgp.55.4.427" @default.
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