FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2163494785> ?p ?o ?g. }

• W2163494785 endingPage "635" @default.
• W2163494785 startingPage "630" @default.
• W2163494785 abstract "Molecular tools, such as real-time nucleic acid sequence-based amplification (NASBA) and PCR, have been developed to detect Trypanosoma brucei parasites in blood for the diagnosis of human African trypanosomiasis (HAT). Despite good sensitivity, these techniques are not implemented in HAT control programs due to the high cost of the equipment, which is unaffordable for laboratories in developing countries where HAT is endemic. In this study, a simplified technique, oligochromatography (OC), was developed for the detection of amplification products of T. brucei 18S rRNA by NASBA. The T. brucei NASBA-OC test has analytical sensitivities of 1 to 10 parasites/ml on nucleic acids extracted from parasite culture and 10 parasites/ml on spiked blood. The test showed no reaction with nontarget pathogens or with blood from healthy controls. Compared to the composite standard applied in the present study, i.e., parasitological confirmation of a HAT case by direct microscopy or by microscopy after concentration of parasites using either a microhematocrit centrifugation technique or a mini-anion-exchange centrifugation technique, NASBA-OC on blood samples had a sensitivity of 73.0% (95% confidence interval, 60 to 83%), while standard expert microscopy had a sensitivity of 57.1% (95% confidence interval, 44 to 69%). On cerebrospinal fluid samples, NASBA-OC had a sensitivity of 88.2% (95% confidence interval, 75 to 95%) and standard microscopy had a sensitivity of 86.2% (95% confidence interval, 64 to 88%). The T. brucei NASBA-OC test developed in this study can be employed in field laboratories, because it does not require a thermocycler; a simple heat block or a water bath maintained at two different temperatures is sufficient for amplification." @default.
• W2163494785 created "2016-06-24" @default.
• W2163494785 creator A5022429594 @default.
• W2163494785 creator A5025445165 @default.
• W2163494785 creator A5044882964 @default.
• W2163494785 creator A5046739881 @default.
• W2163494785 creator A5065059966 @default.
• W2163494785 creator A5087727660 @default.
• W2163494785 date "2009-03-01" @default.
• W2163494785 modified "2023-09-23" @default.
• W2163494785 title "Nucleic Acid Sequence-Based Amplification with Oligochromatography for Detection of <i>Trypanosoma brucei</i> in Clinical Samples" @default.
• W2163494785 cites W1559128470 @default.
• W2163494785 cites W1825078920 @default.
• W2163494785 cites W1965661990 @default.
• W2163494785 cites W1980600975 @default.
• W2163494785 cites W1997609206 @default.
• W2163494785 cites W2004275672 @default.
• W2163494785 cites W2021747987 @default.
• W2163494785 cites W2064185909 @default.
• W2163494785 cites W2067944993 @default.
• W2163494785 cites W2068257075 @default.
• W2163494785 cites W2069436329 @default.
• W2163494785 cites W2070332653 @default.
• W2163494785 cites W2088047783 @default.
• W2163494785 cites W2091849099 @default.
• W2163494785 cites W2099420760 @default.
• W2163494785 cites W2122053924 @default.
• W2163494785 cites W2123509863 @default.
• W2163494785 cites W2137845501 @default.
• W2163494785 cites W2141871601 @default.
• W2163494785 cites W2147973202 @default.
• W2163494785 cites W2160059986 @default.
• W2163494785 cites W2162834085 @default.
• W2163494785 cites W2166229810 @default.
• W2163494785 doi "https://doi.org/10.1128/jcm.01430-08" @default.
• W2163494785 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2650916" @default.
• W2163494785 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19116352" @default.
• W2163494785 hasPublicationYear "2009" @default.
• W2163494785 type Work @default.
• W2163494785 sameAs 2163494785 @default.
• W2163494785 citedByCount "61" @default.
• W2163494785 countsByYear W21634947852012 @default.
• W2163494785 countsByYear W21634947852013 @default.
• W2163494785 countsByYear W21634947852014 @default.
• W2163494785 countsByYear W21634947852015 @default.
• W2163494785 countsByYear W21634947852016 @default.
• W2163494785 countsByYear W21634947852018 @default.
• W2163494785 countsByYear W21634947852019 @default.
• W2163494785 countsByYear W21634947852020 @default.
• W2163494785 countsByYear W21634947852021 @default.
• W2163494785 countsByYear W21634947852022 @default.
• W2163494785 crossrefType "journal-article" @default.
• W2163494785 hasAuthorship W2163494785A5022429594 @default.
• W2163494785 hasAuthorship W2163494785A5025445165 @default.
• W2163494785 hasAuthorship W2163494785A5044882964 @default.
• W2163494785 hasAuthorship W2163494785A5046739881 @default.
• W2163494785 hasAuthorship W2163494785A5065059966 @default.
• W2163494785 hasAuthorship W2163494785A5087727660 @default.
• W2163494785 hasBestOaLocation W21634947851 @default.
• W2163494785 hasConcept C104317684 @default.
• W2163494785 hasConcept C126322002 @default.
• W2163494785 hasConcept C153911025 @default.
• W2163494785 hasConcept C159047783 @default.
• W2163494785 hasConcept C185592680 @default.
• W2163494785 hasConcept C2778143080 @default.
• W2163494785 hasConcept C2778792689 @default.
• W2163494785 hasConcept C2779502636 @default.
• W2163494785 hasConcept C2779695955 @default.
• W2163494785 hasConcept C43617362 @default.
• W2163494785 hasConcept C44249647 @default.
• W2163494785 hasConcept C552990157 @default.
• W2163494785 hasConcept C55493867 @default.
• W2163494785 hasConcept C71924100 @default.
• W2163494785 hasConcept C84148353 @default.
• W2163494785 hasConcept C86803240 @default.
• W2163494785 hasConceptScore W2163494785C104317684 @default.
• W2163494785 hasConceptScore W2163494785C126322002 @default.
• W2163494785 hasConceptScore W2163494785C153911025 @default.
• W2163494785 hasConceptScore W2163494785C159047783 @default.
• W2163494785 hasConceptScore W2163494785C185592680 @default.
• W2163494785 hasConceptScore W2163494785C2778143080 @default.
• W2163494785 hasConceptScore W2163494785C2778792689 @default.
• W2163494785 hasConceptScore W2163494785C2779502636 @default.
• W2163494785 hasConceptScore W2163494785C2779695955 @default.
• W2163494785 hasConceptScore W2163494785C43617362 @default.
• W2163494785 hasConceptScore W2163494785C44249647 @default.
• W2163494785 hasConceptScore W2163494785C552990157 @default.
• W2163494785 hasConceptScore W2163494785C55493867 @default.
• W2163494785 hasConceptScore W2163494785C71924100 @default.
• W2163494785 hasConceptScore W2163494785C84148353 @default.
• W2163494785 hasConceptScore W2163494785C86803240 @default.
• W2163494785 hasIssue "3" @default.
• W2163494785 hasLocation W21634947851 @default.
• W2163494785 hasLocation W21634947852 @default.
• W2163494785 hasLocation W21634947853 @default.
• W2163494785 hasLocation W21634947854 @default.
• W2163494785 hasOpenAccess W2163494785 @default.
• W2163494785 hasPrimaryLocation W21634947851 @default.

• next