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- W2163575737 abstract "Pure phosphatidyl glycerol was obtained from spinach leaves after repeated chromatography on silica columns. Ascertainment of the configuration of the hydrolysis products formed by the action of phospholipases C (EC 3.1.4.3) and D (EC 3.1.4.4) demonstrated that this phospholipid is identical with 1,2-diacyl-glycerol-3-phosphoryl-1-glycerol. Fatty acid analysis of several lipid fractions showed that the Δ3-trans-hexadecenoic acid, present in the leaves, is concentrated almost exclusively in phosphatidyl glycerol. Degradation experiments with phospholipase A (EC 3.1.1.4) showed that this acid is located preferentially at the 2-ester position. A subfractionation of phosphatidyl glycerol was accomplished by thin-layer chromatography on silica plates impregnated with silver nitrate. A breakdown of the two fractions obtained with phospholipase A allowed the recognition of several molecular species, and 1-linolenoyl,2-Δ3-trans-hexadecenoyl-glycerol-3-phosphoryl-1′-glycerol appeared to be the major species. The results were confirmed by hydrolysis of phosphatidyl glycerol with phospholipase C and separation on impregnated adsorbents of the diglycerides formed." @default.
- W2163575737 created "2016-06-24" @default.
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- W2163575737 date "1965-07-01" @default.
- W2163575737 modified "2023-09-25" @default.
- W2163575737 title "Isolation and chemical characterization of phosphatidyl glycerol from spinach leaves" @default.
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- W2163575737 doi "https://doi.org/10.1016/0005-2760(65)90097-4" @default.
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