Matches in SemOpenAlex for { <https://semopenalex.org/work/W2163744897> ?p ?o ?g. }
- W2163744897 endingPage "233" @default.
- W2163744897 startingPage "221" @default.
- W2163744897 abstract "Prenylation of G protein gamma (γ) subunits is necessary for the membrane localization of heterotrimeric G proteins and for functional heterotrimeric G protein coupled receptor (GPCR) signaling. To evaluate GPCR signaling pathways during development, we injected zebrafish embryos with mRNAs encoding Gγ subunits mutated so that they can no longer be prenylated. Low-level expression of these prenylation-deficient Gγ subunits driven either ubiquitously or specifically in the primordial germ cells (PGCs) disrupts GPCR signaling and manifests as a PGC migration defect. This disruption results in a reduction of calcium accumulation in the protrusions of migrating PGCs and a failure of PGCs to directionally migrate. When co-expressed with a prenylation-deficient Gγ, 8 of the 17 wildtype Gγ isoforms individually confer the ability to restore calcium accumulation and directional migration. These results suggest that while the Gγ subunits possess the ability to interact with G Beta (β) proteins, only a subset of wildtype Gγ proteins are stable within PGCs and can interact with key signaling components necessary for PGC migration. This in vivo study highlights the functional redundancy of these signaling components and demonstrates that prenylation-deficient Gγ subunits are an effective tool to investigate the roles of GPCR signaling events during vertebrate development." @default.
- W2163744897 created "2016-06-24" @default.
- W2163744897 creator A5039081372 @default.
- W2163744897 creator A5052999785 @default.
- W2163744897 creator A5068084852 @default.
- W2163744897 creator A5081280205 @default.
- W2163744897 date "2010-02-01" @default.
- W2163744897 modified "2023-10-02" @default.
- W2163744897 title "Prenylation-deficient G protein gamma subunits disrupt GPCR signaling in the zebrafish" @default.
- W2163744897 cites W1482653638 @default.
- W2163744897 cites W1490936260 @default.
- W2163744897 cites W1492894785 @default.
- W2163744897 cites W1497257474 @default.
- W2163744897 cites W1497665885 @default.
- W2163744897 cites W1499914765 @default.
- W2163744897 cites W1511498323 @default.
- W2163744897 cites W1512691892 @default.
- W2163744897 cites W1530195638 @default.
- W2163744897 cites W1532095908 @default.
- W2163744897 cites W1549892775 @default.
- W2163744897 cites W1555420746 @default.
- W2163744897 cites W1591707154 @default.
- W2163744897 cites W1593673222 @default.
- W2163744897 cites W1593806089 @default.
- W2163744897 cites W1601599684 @default.
- W2163744897 cites W1604214492 @default.
- W2163744897 cites W1604834232 @default.
- W2163744897 cites W1646966930 @default.
- W2163744897 cites W1780629085 @default.
- W2163744897 cites W181525583 @default.
- W2163744897 cites W1952953777 @default.
- W2163744897 cites W1963879756 @default.
- W2163744897 cites W1967787845 @default.
- W2163744897 cites W1968809812 @default.
- W2163744897 cites W1969583788 @default.
- W2163744897 cites W1969670014 @default.
- W2163744897 cites W1972211504 @default.
- W2163744897 cites W1976310120 @default.
- W2163744897 cites W1984114573 @default.
- W2163744897 cites W1985741841 @default.
- W2163744897 cites W1985860624 @default.
- W2163744897 cites W1986842713 @default.
- W2163744897 cites W1988744406 @default.
- W2163744897 cites W1994412419 @default.
- W2163744897 cites W2005283218 @default.
- W2163744897 cites W2007282080 @default.
- W2163744897 cites W2017986798 @default.
- W2163744897 cites W2019347876 @default.
- W2163744897 cites W2023106672 @default.
- W2163744897 cites W2025837534 @default.
- W2163744897 cites W2026227107 @default.
- W2163744897 cites W2028396197 @default.
- W2163744897 cites W2028400715 @default.
- W2163744897 cites W2038593831 @default.
- W2163744897 cites W2039360893 @default.
- W2163744897 cites W2039902966 @default.
- W2163744897 cites W2044775506 @default.
- W2163744897 cites W2046197443 @default.
- W2163744897 cites W2050464584 @default.
- W2163744897 cites W2052280808 @default.
- W2163744897 cites W2053188090 @default.
- W2163744897 cites W2054984160 @default.
- W2163744897 cites W2056458954 @default.
- W2163744897 cites W2056965979 @default.
- W2163744897 cites W2059585924 @default.
- W2163744897 cites W2059659204 @default.
- W2163744897 cites W2059843465 @default.
- W2163744897 cites W2061876104 @default.
- W2163744897 cites W2062716507 @default.
- W2163744897 cites W2063379302 @default.
- W2163744897 cites W2064464664 @default.
- W2163744897 cites W2065664139 @default.
- W2163744897 cites W2066984263 @default.
- W2163744897 cites W2070618535 @default.
- W2163744897 cites W2075366797 @default.
- W2163744897 cites W2076049111 @default.
- W2163744897 cites W2079001140 @default.
- W2163744897 cites W2083898995 @default.
- W2163744897 cites W2085668133 @default.
- W2163744897 cites W2086219908 @default.
- W2163744897 cites W2086617442 @default.
- W2163744897 cites W2089627071 @default.
- W2163744897 cites W2091112155 @default.
- W2163744897 cites W2092823346 @default.
- W2163744897 cites W2095420301 @default.
- W2163744897 cites W2102867972 @default.
- W2163744897 cites W2108707790 @default.
- W2163744897 cites W2113557771 @default.
- W2163744897 cites W2118182695 @default.
- W2163744897 cites W2118624685 @default.
- W2163744897 cites W2123272310 @default.
- W2163744897 cites W2125326682 @default.
- W2163744897 cites W2125349610 @default.
- W2163744897 cites W2133682555 @default.
- W2163744897 cites W2150014745 @default.
- W2163744897 cites W2150754101 @default.
- W2163744897 cites W2155271887 @default.
- W2163744897 cites W2156323704 @default.