FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2163971105> ?p ?o ?g. }

• W2163971105 endingPage "26222" @default.
• W2163971105 startingPage "26213" @default.
• W2163971105 abstract "p-Hydroxyphenylacetate (HPA) 3-hydroxylase from Acinetobacter baumannii consists of a reductase component (C(1)) and an oxygenase component (C(2)). C(1) catalyzes the reduction of FMN by NADH to provide FMNH(-) as a substrate for C(2). The rate of reduction of flavin is enhanced ∼20-fold by binding HPA. The N-terminal domain of C(1) is homologous to other flavin reductases, whereas the C-terminal domain (residues 192-315) is similar to MarR, a repressor protein involved in bacterial antibiotic resistance. In this study, three forms of truncated C(1) variants and single site mutation variants of residues Arg-21, Phe-216, Arg-217, Ile-246, and Arg-247 were constructed to investigate the role of the C-terminal domain in regulating C(1). In the absence of HPA, the C(1) variant in which residues 179-315 were removed (t178C(1)) was reduced by NADH and released FMNH(-) at the same rates as wild-type enzyme carries out these functions in the presence of HPA. In contrast, variants with residues 231-315 removed behaved similarly to the wild-type enzyme. Thus, residues 179-230 are involved in repressing the production of FMNH(-) in the absence of HPA. These results are consistent with the C-terminal domain in the wild-type enzyme being an autoinhibitory domain that upon binding the effector HPA undergoes conformational changes to allow faster flavin reduction and release. Most of the single site variants investigated had catalytic properties similar to those of the wild-type enzyme except for the F216A variant, which had a rate of reduction that was not stimulated by HPA. F216A could be involved with HPA binding or in the required conformational change for stimulation of flavin reduction by HPA." @default.
• W2163971105 created "2016-06-24" @default.
• W2163971105 creator A5005497332 @default.
• W2163971105 creator A5015658705 @default.
• W2163971105 creator A5017369266 @default.
• W2163971105 creator A5023125790 @default.
• W2163971105 creator A5023356010 @default.
• W2163971105 creator A5061687760 @default.
• W2163971105 creator A5076264272 @default.
• W2163971105 creator A5084512531 @default.
• W2163971105 date "2012-07-01" @default.
• W2163971105 modified "2023-10-06" @default.
• W2163971105 title "The C-terminal Domain of 4-Hydroxyphenylacetate 3-Hydroxylase from Acinetobacter baumannii Is an Autoinhibitory Domain" @default.
• W2163971105 cites W1516064099 @default.
• W2163971105 cites W1523642899 @default.
• W2163971105 cites W1572779399 @default.
• W2163971105 cites W1619230477 @default.
• W2163971105 cites W1927794787 @default.
• W2163971105 cites W1965160406 @default.
• W2163971105 cites W1974238327 @default.
• W2163971105 cites W1975326331 @default.
• W2163971105 cites W1981378753 @default.
• W2163971105 cites W1983893123 @default.
• W2163971105 cites W1984045227 @default.
• W2163971105 cites W1989002197 @default.
• W2163971105 cites W1990976669 @default.
• W2163971105 cites W1991238273 @default.
• W2163971105 cites W1993681748 @default.
• W2163971105 cites W1995532454 @default.
• W2163971105 cites W1998369828 @default.
• W2163971105 cites W2012580167 @default.
• W2163971105 cites W2015642465 @default.
• W2163971105 cites W2028089689 @default.
• W2163971105 cites W2033330355 @default.
• W2163971105 cites W2035418860 @default.
• W2163971105 cites W2038340006 @default.
• W2163971105 cites W2038881205 @default.
• W2163971105 cites W2041610864 @default.
• W2163971105 cites W2044540564 @default.
• W2163971105 cites W2054284262 @default.
• W2163971105 cites W2055609118 @default.
• W2163971105 cites W2060809301 @default.
• W2163971105 cites W2063910850 @default.
• W2163971105 cites W2068252164 @default.
• W2163971105 cites W2074083103 @default.
• W2163971105 cites W2075024984 @default.
• W2163971105 cites W2081981048 @default.
• W2163971105 cites W2092943003 @default.
• W2163971105 cites W2093060889 @default.
• W2163971105 cites W2094670142 @default.
• W2163971105 cites W2125226304 @default.
• W2163971105 cites W2131174843 @default.
• W2163971105 cites W2134882556 @default.
• W2163971105 cites W2135106844 @default.
• W2163971105 cites W2138262431 @default.
• W2163971105 cites W2139127203 @default.
• W2163971105 cites W2145538498 @default.
• W2163971105 cites W2152301430 @default.
• W2163971105 cites W2158353431 @default.
• W2163971105 cites W2170186817 @default.
• W2163971105 cites W2069721141 @default.
• W2163971105 doi "https://doi.org/10.1074/jbc.m112.354472" @default.
• W2163971105 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3406706" @default.
• W2163971105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22661720" @default.
• W2163971105 hasPublicationYear "2012" @default.
• W2163971105 type Work @default.
• W2163971105 sameAs 2163971105 @default.
• W2163971105 citedByCount "24" @default.
• W2163971105 countsByYear W21639711052013 @default.
• W2163971105 countsByYear W21639711052014 @default.
• W2163971105 countsByYear W21639711052015 @default.
• W2163971105 countsByYear W21639711052016 @default.
• W2163971105 countsByYear W21639711052017 @default.
• W2163971105 countsByYear W21639711052018 @default.
• W2163971105 countsByYear W21639711052019 @default.
• W2163971105 countsByYear W21639711052020 @default.
• W2163971105 countsByYear W21639711052022 @default.
• W2163971105 crossrefType "journal-article" @default.
• W2163971105 hasAuthorship W2163971105A5005497332 @default.
• W2163971105 hasAuthorship W2163971105A5015658705 @default.
• W2163971105 hasAuthorship W2163971105A5017369266 @default.
• W2163971105 hasAuthorship W2163971105A5023125790 @default.
• W2163971105 hasAuthorship W2163971105A5023356010 @default.
• W2163971105 hasAuthorship W2163971105A5061687760 @default.
• W2163971105 hasAuthorship W2163971105A5076264272 @default.
• W2163971105 hasAuthorship W2163971105A5084512531 @default.
• W2163971105 hasBestOaLocation W21639711051 @default.
• W2163971105 hasConcept C104317684 @default.
• W2163971105 hasConcept C134651460 @default.
• W2163971105 hasConcept C143065580 @default.
• W2163971105 hasConcept C181199279 @default.
• W2163971105 hasConcept C185592680 @default.
• W2163971105 hasConcept C197957613 @default.
• W2163971105 hasConcept C207583985 @default.
• W2163971105 hasConcept C2776315533 @default.
• W2163971105 hasConcept C2777637488 @default.
• W2163971105 hasConcept C33093398 @default.
• W2163971105 hasConcept C523546767 @default.

• next