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• W2164078069 abstract "This paper describes in a historical perspective the development of serum-free nutrient media suitable for long-term culturing of nerve tissue. Several disadvantages of the use of serum are discussed, coupled with an acknowledgement that it is not always advisable to replace a routinely used serum-supplemented medium by a chemically defined medium with the expectation of immediate success. Therefore a strategy is given on how to develop a chemically defined medium that is thoroughly tuned to the specific needs of the cell type to be cultured. It is argued that such a medium has several substantial advantages over the use of serum." @default.
• W2164078069 created "2016-06-24" @default.
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• W2164078069 date "1988-01-01" @default.
• W2164078069 modified "2023-09-24" @default.
• W2164078069 title "Development and advantages of serum-free, chemically defined nutrient media for culturing of nerve tissue" @default.
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• W2164078069 doi "https://doi.org/10.1016/0248-4900(88)90116-5" @default.
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