FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2164080508> ?p ?o ?g. }

• W2164080508 endingPage "93" @default.
• W2164080508 startingPage "3886" @default.
• W2164080508 abstract "Two major pathways for repairing DNA double-strand breaks (DSBs) have been identified in mammalian cells, nonhomologous end-joining (NHEJ) and homologous recombination (HR). Inactivation of NHEJ is known to lead to an elevated level of spontaneous and radiation-induced chromosomal rearrangements associated with an increased risk of tumorigenesis. This has raised the idea of a caretaker role for NHEJ. It is, however, not known whether NHEJ itself can also cause rearrangements. To investigate, on the DNA level, the influence of a defect in NHEJ on the formation of genomic rearrangements, we applied an assay based on Southern hybridization that allows the identification and quantification of incorrectly rejoined DSB ends produced by ionizing radiation. After 80 Gy of X-irradiation at a high dose rate (23 Gy/min), wild-type cells repaired 50% of the induced DSBs within 24 h by incorrect rejoining. This frequency of DSB misrejoining is considerably reduced in NHEJ-deficient cells. Low-dose-rate experiments, in which the cells were exposed to 80 Gy over a period of 14 days under repair conditions, led to no detectable misrejoining in wild-type cells but revealed a misrejoining frequency of 10% in NHEJ-deficient cells. This shows that in situations of separated breaks, NHEJ deficiency leads to genomic rearrangements, in agreement with chromosomal studies. However, if multiple DSBs coincide, even wild-type cells form genomic rearrangements frequently. These repair events are absent in Ku80-, DNA-PKcs-, and DNA ligase IV-deficient cells but are present in RAD54(-/-) cells. This strongly suggests that NHEJ has, in addition to its caretaker role, also the potential to effect genomic rearrangements. We propose that it serves as an efficient pathway for rejoining correct break ends in situations of separated breaks but generates genomic rearrangements if DSBs are close in time and space." @default.
• W2164080508 created "2016-06-24" @default.
• W2164080508 creator A5012297088 @default.
• W2164080508 creator A5050401892 @default.
• W2164080508 creator A5078309099 @default.
• W2164080508 creator A5084291865 @default.
• W2164080508 date "2001-05-15" @default.
• W2164080508 modified "2023-10-17" @default.
• W2164080508 title "Radiation-induced genomic rearrangements formed by nonhomologous end-joining of DNA double-strand breaks." @default.
• W2164080508 cites W1518821520 @default.
• W2164080508 cites W1556688508 @default.
• W2164080508 cites W1750064874 @default.
• W2164080508 cites W1905978644 @default.
• W2164080508 cites W1930722859 @default.
• W2164080508 cites W1965336663 @default.
• W2164080508 cites W1971729092 @default.
• W2164080508 cites W1973023456 @default.
• W2164080508 cites W2000912535 @default.
• W2164080508 cites W2005128568 @default.
• W2164080508 cites W2010594589 @default.
• W2164080508 cites W2010901031 @default.
• W2164080508 cites W2012029496 @default.
• W2164080508 cites W2015331561 @default.
• W2164080508 cites W2017145640 @default.
• W2164080508 cites W2019072372 @default.
• W2164080508 cites W2024604197 @default.
• W2164080508 cites W2026457902 @default.
• W2164080508 cites W2031285384 @default.
• W2164080508 cites W2034562158 @default.
• W2164080508 cites W2035088189 @default.
• W2164080508 cites W2038794308 @default.
• W2164080508 cites W2039810557 @default.
• W2164080508 cites W2044874191 @default.
• W2164080508 cites W2047758510 @default.
• W2164080508 cites W2058664192 @default.
• W2164080508 cites W2058800908 @default.
• W2164080508 cites W2059345576 @default.
• W2164080508 cites W2061262165 @default.
• W2164080508 cites W2063060775 @default.
• W2164080508 cites W2072957347 @default.
• W2164080508 cites W2075019783 @default.
• W2164080508 cites W2080152855 @default.
• W2164080508 cites W2083326500 @default.
• W2164080508 cites W2090365348 @default.
• W2164080508 cites W2095360684 @default.
• W2164080508 cites W2095579692 @default.
• W2164080508 cites W2102603170 @default.
• W2164080508 cites W2103770333 @default.
• W2164080508 cites W2110177038 @default.
• W2164080508 cites W2112823568 @default.
• W2164080508 cites W2113418340 @default.
• W2164080508 cites W2119103734 @default.
• W2164080508 cites W2119125321 @default.
• W2164080508 cites W2120755353 @default.
• W2164080508 cites W2127414222 @default.
• W2164080508 cites W2129971649 @default.
• W2164080508 cites W2130728712 @default.
• W2164080508 cites W2133323814 @default.
• W2164080508 cites W2137347518 @default.
• W2164080508 cites W2139554694 @default.
• W2164080508 cites W2144512576 @default.
• W2164080508 cites W2150414930 @default.
• W2164080508 cites W2150862729 @default.
• W2164080508 cites W2160077005 @default.
• W2164080508 cites W2160089420 @default.
• W2164080508 cites W2161035053 @default.
• W2164080508 cites W2161452450 @default.
• W2164080508 cites W2165579893 @default.
• W2164080508 cites W2166663643 @default.
• W2164080508 cites W2168809955 @default.
• W2164080508 cites W2172576802 @default.
• W2164080508 cites W2318222641 @default.
• W2164080508 cites W2328854054 @default.
• W2164080508 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11358801" @default.
• W2164080508 hasPublicationYear "2001" @default.
• W2164080508 type Work @default.
• W2164080508 sameAs 2164080508 @default.
• W2164080508 citedByCount "53" @default.
• W2164080508 countsByYear W21640805082012 @default.
• W2164080508 countsByYear W21640805082013 @default.
• W2164080508 countsByYear W21640805082015 @default.
• W2164080508 countsByYear W21640805082016 @default.
• W2164080508 countsByYear W21640805082017 @default.
• W2164080508 countsByYear W21640805082018 @default.
• W2164080508 countsByYear W21640805082020 @default.
• W2164080508 countsByYear W21640805082021 @default.
• W2164080508 countsByYear W21640805082022 @default.
• W2164080508 countsByYear W21640805082023 @default.
• W2164080508 crossrefType "journal-article" @default.
• W2164080508 hasAuthorship W2164080508A5012297088 @default.
• W2164080508 hasAuthorship W2164080508A5050401892 @default.
• W2164080508 hasAuthorship W2164080508A5078309099 @default.
• W2164080508 hasAuthorship W2164080508A5084291865 @default.
• W2164080508 hasConcept C102744134 @default.
• W2164080508 hasConcept C104317684 @default.
• W2164080508 hasConcept C121608353 @default.
• W2164080508 hasConcept C122917832 @default.
• W2164080508 hasConcept C134935766 @default.

• next