Matches in SemOpenAlex for { <https://semopenalex.org/work/W2164094488> ?p ?o ?g. }
- W2164094488 endingPage "74" @default.
- W2164094488 startingPage "65" @default.
- W2164094488 abstract "Abstract Human CD8 is a T cell coreceptor, which binds to pHLA I and plays a pivotal role in the activation of cytotoxic T lymphocytes. Soluble recombinant CD8 αα has been shown to antagonize T cell activation, both in vitro and in vivo. However, because of a very low affinity for pHLA I, high concentrations of soluble CD8 αα are required for efficient inhibition. Based upon our knowledge of the wild‐type CD8/pHLA I structure, we have designed and produced a mutated form of soluble CD8 αα that binds to pHLA I with approximately fourfold higher affinity. We have characterized the binding of the high affinity CD8 mutant using surface plasmon resonance and determined its structure at 2.1 Å resolution using X‐ray crystallography. The analysis of this structure suggests that the higher affinity is achieved by providing a larger side chain that allows for an optimal contact to be made between the HLA α3 loop and the mutated CDR‐like loops of CD8. Proteins 2007. © 2007 Wiley‐Liss, Inc." @default.
- W2164094488 created "2016-06-24" @default.
- W2164094488 creator A5009801938 @default.
- W2164094488 creator A5015290004 @default.
- W2164094488 creator A5015873777 @default.
- W2164094488 creator A5016792406 @default.
- W2164094488 creator A5022694581 @default.
- W2164094488 creator A5029346258 @default.
- W2164094488 creator A5046216629 @default.
- W2164094488 creator A5050010614 @default.
- W2164094488 creator A5063476882 @default.
- W2164094488 creator A5069971181 @default.
- W2164094488 date "2007-01-22" @default.
- W2164094488 modified "2023-10-17" @default.
- W2164094488 title "Computational design and crystal structure of an enhanced affinity mutant human CD8 αα coreceptor" @default.
- W2164094488 cites W133369315 @default.
- W2164094488 cites W1497992707 @default.
- W2164094488 cites W1521451515 @default.
- W2164094488 cites W1815116283 @default.
- W2164094488 cites W1977478904 @default.
- W2164094488 cites W2000317966 @default.
- W2164094488 cites W2001641653 @default.
- W2164094488 cites W2008384204 @default.
- W2164094488 cites W2010090839 @default.
- W2164094488 cites W2011503659 @default.
- W2164094488 cites W2013083986 @default.
- W2164094488 cites W2023051297 @default.
- W2164094488 cites W2024441858 @default.
- W2164094488 cites W2027408247 @default.
- W2164094488 cites W2031011895 @default.
- W2164094488 cites W2037893240 @default.
- W2164094488 cites W2038023416 @default.
- W2164094488 cites W2038840577 @default.
- W2164094488 cites W2043429268 @default.
- W2164094488 cites W2059637973 @default.
- W2164094488 cites W2060165194 @default.
- W2164094488 cites W2062216934 @default.
- W2164094488 cites W2065430316 @default.
- W2164094488 cites W2066437704 @default.
- W2164094488 cites W2075000980 @default.
- W2164094488 cites W2078551708 @default.
- W2164094488 cites W2080476827 @default.
- W2164094488 cites W2085106063 @default.
- W2164094488 cites W2094513856 @default.
- W2164094488 cites W2102903171 @default.
- W2164094488 cites W2111480581 @default.
- W2164094488 cites W2118337211 @default.
- W2164094488 cites W2121503187 @default.
- W2164094488 cites W2148426299 @default.
- W2164094488 cites W2162166182 @default.
- W2164094488 cites W2168213821 @default.
- W2164094488 cites W2762980752 @default.
- W2164094488 cites W4235220096 @default.
- W2164094488 doi "https://doi.org/10.1002/prot.21176" @default.
- W2164094488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17243170" @default.
- W2164094488 hasPublicationYear "2007" @default.
- W2164094488 type Work @default.
- W2164094488 sameAs 2164094488 @default.
- W2164094488 citedByCount "29" @default.
- W2164094488 countsByYear W21640944882012 @default.
- W2164094488 countsByYear W21640944882013 @default.
- W2164094488 countsByYear W21640944882014 @default.
- W2164094488 countsByYear W21640944882015 @default.
- W2164094488 countsByYear W21640944882016 @default.
- W2164094488 countsByYear W21640944882017 @default.
- W2164094488 countsByYear W21640944882018 @default.
- W2164094488 countsByYear W21640944882020 @default.
- W2164094488 countsByYear W21640944882022 @default.
- W2164094488 countsByYear W21640944882023 @default.
- W2164094488 crossrefType "journal-article" @default.
- W2164094488 hasAuthorship W2164094488A5009801938 @default.
- W2164094488 hasAuthorship W2164094488A5015290004 @default.
- W2164094488 hasAuthorship W2164094488A5015873777 @default.
- W2164094488 hasAuthorship W2164094488A5016792406 @default.
- W2164094488 hasAuthorship W2164094488A5022694581 @default.
- W2164094488 hasAuthorship W2164094488A5029346258 @default.
- W2164094488 hasAuthorship W2164094488A5046216629 @default.
- W2164094488 hasAuthorship W2164094488A5050010614 @default.
- W2164094488 hasAuthorship W2164094488A5063476882 @default.
- W2164094488 hasAuthorship W2164094488A5069971181 @default.
- W2164094488 hasConcept C104317684 @default.
- W2164094488 hasConcept C106847996 @default.
- W2164094488 hasConcept C12554922 @default.
- W2164094488 hasConcept C143065580 @default.
- W2164094488 hasConcept C147483822 @default.
- W2164094488 hasConcept C154317977 @default.
- W2164094488 hasConcept C155672457 @default.
- W2164094488 hasConcept C167672396 @default.
- W2164094488 hasConcept C171250308 @default.
- W2164094488 hasConcept C185592680 @default.
- W2164094488 hasConcept C192562407 @default.
- W2164094488 hasConcept C202751555 @default.
- W2164094488 hasConcept C40767141 @default.
- W2164094488 hasConcept C54355233 @default.
- W2164094488 hasConcept C55493867 @default.
- W2164094488 hasConcept C86803240 @default.
- W2164094488 hasConcept C95444343 @default.
- W2164094488 hasConceptScore W2164094488C104317684 @default.