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- W2164112899 abstract "Recently, many small non-coding RNAs (sRNAs) with important regulatory roles have been identified in bacteria. As their eukaryotic counterparts, a major class of bacterial trans -encoded sRNAs acts by basepairing with target mRNAs, resulting in changes in translation and stability of the mRNA. RNA interference (RNAi) has become a powerful gene silencing tool in eukaryotes. However, such an effective RNA silencing tool remains to be developed for prokaryotes. In this study, we described first the use of artificial trans -encoded sRNAs (atsRNAs) for specific gene silencing in bacteria. Based on the common structural characteristics of natural sRNAs in Gram-negative bacteria, we developed the designing principle of atsRNA. Most of the atsRNAs effectively suppressed the expression of exogenous EGFP gene and endogenous uidA gene in Escherichia coli . Further studies demonstrated that the mRNA base pairing region and AU rich Hfq binding site were crucial for the activity of atsRNA. The atsRNA-mediated gene silencing was Hfq dependent. The atsRNAs led to gene silencing and RNase E dependent degradation of target mRNA. We also designed a series of atsRNAs which targeted the toxic genes in Staphyloccocus aureus , but found no significant interfering effect. We established an effective method for specific gene silencing in Gram-negative bacteria." @default.
- W2164112899 created "2016-06-24" @default.
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- W2164112899 date "2011-02-03" @default.
- W2164112899 modified "2023-09-23" @default.
- W2164112899 title "Artificial trans -encoded small non-coding RNAs specifically silence the selected gene expression in bacteria" @default.
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- W2164112899 doi "https://doi.org/10.1093/nar/gkr034" @default.
- W2164112899 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3082891" @default.
- W2164112899 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21296758" @default.
- W2164112899 hasPublicationYear "2011" @default.
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