FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2164301277> ?p ?o ?g. }

• W2164301277 endingPage "5110" @default.
• W2164301277 startingPage "5100" @default.
• W2164301277 abstract "An intI-targeted PCR assay was optimized to evaluate the frequency of partial class 2-like integrases relative to putative, environmental IntI elements in clone libraries generated from 17 samples that included various terrestrial, marine, and deep-sea habitats with different exposures to human influence. We identified 169 unique IntI phylotypes (< or =98% amino acid identity) relative to themselves and with respect to those previously described. Among these, six variants showed an undescribed, extended, IntI-specific additional domain. A connection between human influence and the dominance of IntI-2-like variants was also observed. IntI phylotypes 80 to 99% identical to class 2 integrases comprised approximately 70 to 100% (n = 65 to 87) of the IntI elements detected in samples with a high input of fecal waste, whereas IntI2-like sequences were undetected in undisturbed settings and poorly represented (1 to 10%; n = 40 to 79) in environments with moderate or no recent fecal or anthropogenic impact. Eleven partial IntI2-like sequences lacking the signature ochre 179 codon were found among samples of biosolids and agricultural soil supplemented with swine manure, indicating a wider distribution of potentially functional IntI2 variants than previously reported. To evaluate IntI2 distribution patterns beyond the usual hosts, namely, the Enterobacteriaceae, we coupled PCR assays targeted at intI and 16S rRNA loci to G+C fractionation of total DNA extracted from manured cropland. IntI2-like sequences and 16S rRNA phylotypes related to Firmicutes (Clostridium and Bacillus) and Bacteroidetes (Chitinophaga and Sphingobacterium) dominated a low-G+C fraction ( approximately 40 to 45%), suggesting that these groups could be important IntI2 hosts in manured soil. Moreover, G+G fractionation uncovered an additional set of 36 novel IntI phylotypes (< or =98% amino acid identity) undetected in bulk DNA and revealed the prevalence of potentially functional IntI2 variants in the low-G+C fraction." @default.
• W2164301277 created "2016-06-24" @default.
• W2164301277 creator A5003288277 @default.
• W2164301277 creator A5012175598 @default.
• W2164301277 creator A5041807449 @default.
• W2164301277 creator A5053281874 @default.
• W2164301277 creator A5057348487 @default.
• W2164301277 date "2009-08-01" @default.
• W2164301277 modified "2023-10-13" @default.
• W2164301277 title "Worldwide Prevalence of Class 2 Integrases outside the Clinical Setting Is Associated with Human Impact" @default.
• W2164301277 cites W1882301353 @default.
• W2164301277 cites W1967850793 @default.
• W2164301277 cites W1972469893 @default.
• W2164301277 cites W1972544556 @default.
• W2164301277 cites W1980606854 @default.
• W2164301277 cites W1988740874 @default.
• W2164301277 cites W1997154183 @default.
• W2164301277 cites W1997873483 @default.
• W2164301277 cites W1998050089 @default.
• W2164301277 cites W2002645773 @default.
• W2164301277 cites W2004589024 @default.
• W2164301277 cites W2007785727 @default.
• W2164301277 cites W2011187567 @default.
• W2164301277 cites W2011210667 @default.
• W2164301277 cites W2017592299 @default.
• W2164301277 cites W2019075392 @default.
• W2164301277 cites W2022772561 @default.
• W2164301277 cites W2024236935 @default.
• W2164301277 cites W2030735422 @default.
• W2164301277 cites W2040438773 @default.
• W2164301277 cites W2040954358 @default.
• W2164301277 cites W2045318821 @default.
• W2164301277 cites W2053286804 @default.
• W2164301277 cites W2055251926 @default.
• W2164301277 cites W2058729834 @default.
• W2164301277 cites W2060954415 @default.
• W2164301277 cites W2071463866 @default.
• W2164301277 cites W2073332363 @default.
• W2164301277 cites W2076240605 @default.
• W2164301277 cites W2084207931 @default.
• W2164301277 cites W2096898012 @default.
• W2164301277 cites W2097382368 @default.
• W2164301277 cites W2101326223 @default.
• W2164301277 cites W2102259392 @default.
• W2164301277 cites W2102274379 @default.
• W2164301277 cites W2102917628 @default.
• W2164301277 cites W2103784576 @default.
• W2164301277 cites W2104688104 @default.
• W2164301277 cites W2105046957 @default.
• W2164301277 cites W2109867978 @default.
• W2164301277 cites W2111775329 @default.
• W2164301277 cites W2118701853 @default.
• W2164301277 cites W2118968708 @default.
• W2164301277 cites W2119416861 @default.
• W2164301277 cites W2123109437 @default.
• W2164301277 cites W2125121305 @default.
• W2164301277 cites W2126884217 @default.
• W2164301277 cites W2131988453 @default.
• W2164301277 cites W2134848381 @default.
• W2164301277 cites W2147260319 @default.
• W2164301277 cites W2155285716 @default.
• W2164301277 cites W2157069400 @default.
• W2164301277 cites W2158714788 @default.
• W2164301277 cites W2159171451 @default.
• W2164301277 cites W2159869703 @default.
• W2164301277 cites W2159949205 @default.
• W2164301277 cites W2160000759 @default.
• W2164301277 cites W2161589983 @default.
• W2164301277 cites W2161620968 @default.
• W2164301277 cites W2161721664 @default.
• W2164301277 cites W2162825747 @default.
• W2164301277 cites W2164202787 @default.
• W2164301277 cites W2164956104 @default.
• W2164301277 cites W2165012009 @default.
• W2164301277 cites W2167350133 @default.
• W2164301277 cites W2167965168 @default.
• W2164301277 cites W4210400672 @default.
• W2164301277 doi "https://doi.org/10.1128/aem.00133-09" @default.
• W2164301277 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2725508" @default.
• W2164301277 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19502434" @default.
• W2164301277 hasPublicationYear "2009" @default.
• W2164301277 type Work @default.
• W2164301277 sameAs 2164301277 @default.
• W2164301277 citedByCount "31" @default.
• W2164301277 countsByYear W21643012772012 @default.
• W2164301277 countsByYear W21643012772013 @default.
• W2164301277 countsByYear W21643012772014 @default.
• W2164301277 countsByYear W21643012772015 @default.
• W2164301277 countsByYear W21643012772016 @default.
• W2164301277 countsByYear W21643012772017 @default.
• W2164301277 countsByYear W21643012772018 @default.
• W2164301277 countsByYear W21643012772020 @default.
• W2164301277 countsByYear W21643012772021 @default.
• W2164301277 countsByYear W21643012772022 @default.
• W2164301277 countsByYear W21643012772023 @default.
• W2164301277 crossrefType "journal-article" @default.
• W2164301277 hasAuthorship W2164301277A5003288277 @default.
• W2164301277 hasAuthorship W2164301277A5012175598 @default.

• next