FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2164389678> ?p ?o ?g. }

• W2164389678 abstract "Background: The irritable bowel syndrome (IBS) is one of the most prevalent disorders and affects about 15% of the Swedish population. Patients with IBS suffer from abdominal pain and disturbed bowel function. Despite being so common, little is known about causality in IBS. Patients with IBS have been found to exhibit low-grade inflammation of nerve plexuses in the gut. The driving force for observed immune activation is yet unknown. Aim of the thesis: To assess if bacteria may have a role in the pathogenesis of IBS. This was addressed using different models. The first was to investigate if Chlamydia antigens were present in the small bowel of patients with severe IBS. Then the interaction between Chlamydia and enteroendocrine cells (EEC) in vitro using enteroendocrine cell lines was studied and finally the composition of small bowel mucosa-associated microbiota was explored in patients with IBS and healthy controls using pyrotag sequencing. Material and methods: In Study I full-thickness jejunum biopsies and mucosa biopsies from the duodenum and the jejunum from patients with severe IBS and healthy controls were investigated with immunofluorescence for chlamydial antigens. In studies II and III two different human enteroendocrine cell lines were studied: LCC-18 from a neuroendocrine colonic tumour and CNDT-2 from a small intestinal carcinoid. Cell lines were infected with C. trachomatis serovar LGV II strain 434. Penicillin G was used for inducing persistent infection. The ultrastructure of infected cells was studied using transmission electron microscopy and immunofluorescence and we used RTPCR analysis and microarray analysis (Affymetrix GeneChip®) for studying changes in gene expression at different stages of infection. In Study IV capsule biopsies from the jejunum of patients with IBS and healthy volunteers were studied using barcoded 454-pyrosequencing to determine the composition of microbial communities in the upper small bowel. Results: Chlamydia LPS was detected in enteroendocrine cells of the mucosa or subepithelial macrophages in 89% of patients with IBS, but in only 14% of healthy controls (p < 0.001) and 79% of LPS-positive biopsies were also positive for C. trachomatis major outer membrane protein. The cell line experiments showed that both cell lines could be infected with C. trachomatis yielding productive infection and persistence could be induced using penicillin G. The cellular distribution of serotonin and chromogranin A was altered by infection from a cytoplasmatic distribution to a location mostly in chlamydial inclusions. Significant differences in the gene transcription levels between persistently infected and non-infected cells were found in 10 genes coding for different solute carrier transporters (SLC) and 5 genes related to endocrine function (GABARAPL1, GRIP1, DRD2, SYT5 and SYT7). Study IV showed no major difference in small bowel mucosa-associated microbiota between patients with IBS and healthy controls. Conclusions: Study I introduced the novel concept that infection with C. trachomatis might be involved in the pathogenesis of IBS. In vitro studies confirmed that such an infection affects enteroendocrine cell function. The luminal flora of the small bowel was not identified as a host factor for developing IBS. Key-words: Chlamydia, irritable bowel syndrome, enteroendocrine cells, immunofluorescence, microarray, pyrotag sequencing, small bowel microbiota LIST OF PUBLICATIONS I. Dlugosz A, Tornblom H, Mohammadian G, Morgan G, Veress B, Edvinsson B, Sandstrom G, Lindberg G. Chlamydia trachomatis antigens in enteroendocrine cells and macrophages of the small bowel in patients with severe irritable bowel syndrome. BMC Gastroenterol. 2010; 10: 19. II. Dlugosz A, Zakikhany K, Muschiol S, Hultenby K, Lindberg G. Infection of human enteroendocrine cells with Chlamydia trachomatis: a possible model for pathogenesis in irritable bowel syndrome. Neurogastroenterol Motil. 2011; 23: 928-34. III. Dlugosz A, Muschiol S, Zakikhany K, D'Amato M, Lindberg G. Gene expression analysis of Chlamydia trachomatis infected human enteroendocrine cells. (Submitted for publication) IV. Dlugosz A, Lundin E, Zakikhany K, Sandstrom G, Engstrand L, Lindberg G. Small bowel microbiota in patients with irritable bowel syndrome and healthy controls – a pyrosequencing study. (Submitted for publication) Related publication Zucchelli M, Camilleri M, Nixon Andreasson A, Bresso F, Dlugosz A, Halfvarson J, Torkvist L, Schmidt PT, Karling P, Ohlsson B, Duerr RH, Simren M, Lindberg G, Agreus L, Carlson P, Zinsmeister AR, D'Amato M. Association of TNFSF15 polymorphism with irritable bowel syndrome. Gut. 2011 Jun 2 doi:10.1136/gut.2011.241877" @default.
• W2164389678 created "2016-06-24" @default.
• W2164389678 creator A5077928388 @default.
• W2164389678 date "2011-11-01" @default.
• W2164389678 modified "2023-09-24" @default.
• W2164389678 title "Towards a bacterial origin of Irritable Bowel Syndrome" @default.
• W2164389678 cites W1483462250 @default.
• W2164389678 cites W1512522653 @default.
• W2164389678 cites W1533335653 @default.
• W2164389678 cites W1546952499 @default.
• W2164389678 cites W1589856512 @default.
• W2164389678 cites W1848365949 @default.
• W2164389678 cites W1878895457 @default.
• W2164389678 cites W1910535879 @default.
• W2164389678 cites W1965922372 @default.
• W2164389678 cites W1966213995 @default.
• W2164389678 cites W1970178265 @default.
• W2164389678 cites W1970464559 @default.
• W2164389678 cites W1974175765 @default.
• W2164389678 cites W1976118863 @default.
• W2164389678 cites W1977092679 @default.
• W2164389678 cites W1977282936 @default.
• W2164389678 cites W1979431125 @default.
• W2164389678 cites W1982602254 @default.
• W2164389678 cites W1982930075 @default.
• W2164389678 cites W1986138737 @default.
• W2164389678 cites W1990766250 @default.
• W2164389678 cites W1993725963 @default.
• W2164389678 cites W1995000861 @default.
• W2164389678 cites W1995857191 @default.
• W2164389678 cites W1996161146 @default.
• W2164389678 cites W1999154644 @default.
• W2164389678 cites W1999359264 @default.
• W2164389678 cites W2002962269 @default.
• W2164389678 cites W2003617540 @default.
• W2164389678 cites W2005399514 @default.
• W2164389678 cites W2007116043 @default.
• W2164389678 cites W2007435811 @default.
• W2164389678 cites W2007666404 @default.
• W2164389678 cites W2012442346 @default.
• W2164389678 cites W2015025080 @default.
• W2164389678 cites W2015514143 @default.
• W2164389678 cites W2017093118 @default.
• W2164389678 cites W2020925273 @default.
• W2164389678 cites W2022783819 @default.
• W2164389678 cites W2024210565 @default.
• W2164389678 cites W2024601462 @default.
• W2164389678 cites W2026565706 @default.
• W2164389678 cites W2028889492 @default.
• W2164389678 cites W2028890830 @default.
• W2164389678 cites W2032230795 @default.
• W2164389678 cites W2034157397 @default.
• W2164389678 cites W2035206061 @default.
• W2164389678 cites W2038437697 @default.
• W2164389678 cites W2038838030 @default.
• W2164389678 cites W2039822351 @default.
• W2164389678 cites W2040198542 @default.
• W2164389678 cites W2041136224 @default.
• W2164389678 cites W2043239636 @default.
• W2164389678 cites W2046806891 @default.
• W2164389678 cites W2047172408 @default.
• W2164389678 cites W2053593613 @default.
• W2164389678 cites W2056246809 @default.
• W2164389678 cites W2056557244 @default.
• W2164389678 cites W2059270078 @default.
• W2164389678 cites W2059488695 @default.
• W2164389678 cites W2059521692 @default.
• W2164389678 cites W2060120567 @default.
• W2164389678 cites W2063053656 @default.
• W2164389678 cites W2063653508 @default.
• W2164389678 cites W2064201279 @default.
• W2164389678 cites W2066452478 @default.
• W2164389678 cites W2069225280 @default.
• W2164389678 cites W2078814736 @default.
• W2164389678 cites W2079335614 @default.
• W2164389678 cites W2081098333 @default.
• W2164389678 cites W2086780780 @default.
• W2164389678 cites W2087717115 @default.
• W2164389678 cites W2087956925 @default.
• W2164389678 cites W2089752133 @default.
• W2164389678 cites W2091090762 @default.
• W2164389678 cites W2097566770 @default.
• W2164389678 cites W2097707664 @default.
• W2164389678 cites W2104890139 @default.
• W2164389678 cites W2105445407 @default.
• W2164389678 cites W2105522288 @default.
• W2164389678 cites W2105640447 @default.
• W2164389678 cites W2110065044 @default.
• W2164389678 cites W2113706912 @default.
• W2164389678 cites W2114104545 @default.
• W2164389678 cites W2114455930 @default.
• W2164389678 cites W2117813650 @default.
• W2164389678 cites W2118173539 @default.
• W2164389678 cites W2118451157 @default.
• W2164389678 cites W2124821571 @default.
• W2164389678 cites W2125123579 @default.
• W2164389678 cites W2125826054 @default.
• W2164389678 cites W2129297365 @default.
• W2164389678 cites W2130488316 @default.
• W2164389678 cites W2132703507 @default.

• next