FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2164401340> ?p ?o ?g. }

• W2164401340 endingPage "3598" @default.
• W2164401340 startingPage "3592" @default.
• W2164401340 abstract "LPS from bacteria can result in the development of sepsis syndrome and acute lung injury. Although acute exposure to endotoxin primes leukocytes for enhanced synthesis of leukotrienes (LT), little is known about the effect of chronic exposure. Therefore, we determined the effect of prolonged LPS treatment on 5-lipoxygenase (5-LO) metabolism of arachidonic acid in alveolar macrophages (AM) and in peripheral blood monocytes. Pretreatment of AM with LPS caused time- and dose-dependent suppression of LT synthetic capacity. LPS pretreatment failed to inhibit arachidonic acid (AA) release. The fact that LPS inhibited LT synthesis from endogenous AA more than from exogenous AA suggested an effect on 5-LO-activating protein (FLAP). In addition, an inhibitory effect of LPS treatment on AM 5-LO activity was suggested by cell-free 5-LO enzyme assay. No effect on the expression of either 5-LO or FLAP proteins was observed. New protein synthesis was necessary for LPS-induced reduction of 5-LO metabolism in AM, and immunoblotting demonstrated marked induction of NO synthase (NOS). Inhibition by LPS was reproduced by an NO donor and was abrogated by inhibitors of constitutive and inducible NOS. Compared with AM, peripheral blood monocytes exhibited no suppression by LPS of 5-LO metabolism and no induction of inducible NOS. We conclude that prolonged exposure to LPS impairs AM 5-LO metabolism by NO-mediated suppression of both 5-LO and FLAP function. Because LT contribute to antimicrobial defense, this down-regulation of 5-LO metabolism may contribute to the increased susceptibility to pneumonia in patients following sepsis." @default.
• W2164401340 created "2016-06-24" @default.
• W2164401340 creator A5007817127 @default.
• W2164401340 creator A5028601000 @default.
• W2164401340 creator A5084604101 @default.
• W2164401340 date "2000-10-01" @default.
• W2164401340 modified "2023-10-14" @default.
• W2164401340 title "Prolonged Exposure to Lipopolysaccharide Inhibits Macrophage 5-Lipoxygenase Metabolism Via Induction of Nitric Oxide Synthesis" @default.
• W2164401340 cites W1496063181 @default.
• W2164401340 cites W1509307264 @default.
• W2164401340 cites W1541866678 @default.
• W2164401340 cites W1573038981 @default.
• W2164401340 cites W1587123510 @default.
• W2164401340 cites W1937827645 @default.
• W2164401340 cites W1967245379 @default.
• W2164401340 cites W1967903910 @default.
• W2164401340 cites W1976329278 @default.
• W2164401340 cites W1979772437 @default.
• W2164401340 cites W1980832959 @default.
• W2164401340 cites W1982523785 @default.
• W2164401340 cites W1985419505 @default.
• W2164401340 cites W1998636602 @default.
• W2164401340 cites W2004351423 @default.
• W2164401340 cites W2012438626 @default.
• W2164401340 cites W2014768234 @default.
• W2164401340 cites W2015246822 @default.
• W2164401340 cites W2016869710 @default.
• W2164401340 cites W2019385568 @default.
• W2164401340 cites W2023834383 @default.
• W2164401340 cites W2028534290 @default.
• W2164401340 cites W2029251290 @default.
• W2164401340 cites W2031212351 @default.
• W2164401340 cites W2038398469 @default.
• W2164401340 cites W2038763722 @default.
• W2164401340 cites W2042322531 @default.
• W2164401340 cites W2043720899 @default.
• W2164401340 cites W2054510493 @default.
• W2164401340 cites W2055962814 @default.
• W2164401340 cites W2058747380 @default.
• W2164401340 cites W2060588556 @default.
• W2164401340 cites W2061167474 @default.
• W2164401340 cites W2067139867 @default.
• W2164401340 cites W2069676891 @default.
• W2164401340 cites W2071037043 @default.
• W2164401340 cites W2072835354 @default.
• W2164401340 cites W2073590270 @default.
• W2164401340 cites W2074989201 @default.
• W2164401340 cites W2078420566 @default.
• W2164401340 cites W2079409513 @default.
• W2164401340 cites W2079679312 @default.
• W2164401340 cites W2081432319 @default.
• W2164401340 cites W2081462803 @default.
• W2164401340 cites W2089747387 @default.
• W2164401340 cites W2092345216 @default.
• W2164401340 cites W2093490808 @default.
• W2164401340 cites W2093585097 @default.
• W2164401340 cites W2111144017 @default.
• W2164401340 cites W2120179212 @default.
• W2164401340 cites W2131368450 @default.
• W2164401340 cites W2133516569 @default.
• W2164401340 cites W2151702529 @default.
• W2164401340 cites W2170867229 @default.
• W2164401340 cites W2170910387 @default.
• W2164401340 cites W2325612100 @default.
• W2164401340 cites W2335787271 @default.
• W2164401340 cites W2341091473 @default.
• W2164401340 cites W2406801309 @default.
• W2164401340 cites W2412199294 @default.
• W2164401340 cites W2416189624 @default.
• W2164401340 cites W4250845720 @default.
• W2164401340 cites W4298273896 @default.
• W2164401340 doi "https://doi.org/10.4049/jimmunol.165.7.3592" @default.
• W2164401340 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11034360" @default.
• W2164401340 hasPublicationYear "2000" @default.
• W2164401340 type Work @default.
• W2164401340 sameAs 2164401340 @default.
• W2164401340 citedByCount "67" @default.
• W2164401340 countsByYear W21644013402012 @default.
• W2164401340 countsByYear W21644013402014 @default.
• W2164401340 countsByYear W21644013402015 @default.
• W2164401340 countsByYear W21644013402016 @default.
• W2164401340 countsByYear W21644013402018 @default.
• W2164401340 countsByYear W21644013402019 @default.
• W2164401340 countsByYear W21644013402020 @default.
• W2164401340 countsByYear W21644013402021 @default.
• W2164401340 countsByYear W21644013402022 @default.
• W2164401340 countsByYear W21644013402023 @default.
• W2164401340 crossrefType "journal-article" @default.
• W2164401340 hasAuthorship W2164401340A5007817127 @default.
• W2164401340 hasAuthorship W2164401340A5028601000 @default.
• W2164401340 hasAuthorship W2164401340A5084604101 @default.
• W2164401340 hasBestOaLocation W21644013401 @default.
• W2164401340 hasConcept C134018914 @default.
• W2164401340 hasConcept C16613235 @default.
• W2164401340 hasConcept C181199279 @default.
• W2164401340 hasConcept C185592680 @default.
• W2164401340 hasConcept C202751555 @default.
• W2164401340 hasConcept C203014093 @default.

• next