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- W2164419004 startingPage "4052" @default.
- W2164419004 abstract "We have demonstrated that genes from Ustilago maydis can be cloned by direct complementation of mutants through the use of genomic libraries made in a high-frequency transformation vector. We isolated a gene involved in amino acid biosynthesis as an illustrative example and showed that integrative and one-step disruption methods can be used to create null mutations in the chromosomal copy of the gene by homologous recombination. The results of this investigation make it clear that one-step gene disruption will be of general utility in investigations of U. maydis, since simple, precise replacement of the sequence under study was readily achieved." @default.
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- W2164419004 date "1989-09-01" @default.
- W2164419004 modified "2023-09-27" @default.
- W2164419004 title "Cloning and disruption of Ustilago maydis genes." @default.
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- W2164419004 doi "https://doi.org/10.1128/mcb.9.9.4052" @default.
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