Matches in SemOpenAlex for { <https://semopenalex.org/work/W2164421581> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2164421581 endingPage "3237" @default.
- W2164421581 startingPage "3227" @default.
- W2164421581 abstract "Conversion of leucine to isovaleric acid by Clostridium bifermentans is achieved by the action of at least two enzymes. One is a transaminase producing alpha-ketoisocaproic acid, which was purified 30-fold from osmotic lysates of late-exponential phase cells by repeated chromatography on DEAE-Sepharose C16B and Sephacryl S300: this represented a 147-fold purification of activity found in sonically disrupted cells. This enzyme had an apparent molecular weight of approximately 190000 and was composed of six identically sized sub-units (molecular weight 31000 +/- 1000). Transamination required pyridoxal phosphate and pyruvate and was optimal at pH 8.6; the apparent Km for leucine was 7.0 mM. Activity was totally inhibited by 1 mM-p-chloromercuribenzoate and partially inhibited by other thiol reagents. The second enzyme decarboxylated alpha-ketoisocaproic acid to form isovaleric acid and was also partially purified by chromatography on DEAE-Sepharose C16B and Sephacryl S300. It has an apparent molecular weight of 240000 and required FAD and coenzyme A for activity; the Km for alpha-ketoisocaproic acid was 4.2 mM and activity was optimal around pH 8.0. This enzyme was a flavoprotein with absorption maxima at 280, 320 and 400 nm, and a fluorescent maximum at 500 nm. The prosthetic group, FAD, dissociated from the protein during purification resulting in an inactive apoenzyme which was only partially re-activated by FAD. Activity was completely inhibited by several thiol reagents tested at 1 mM." @default.
- W2164421581 created "2016-06-24" @default.
- W2164421581 creator A5000714877 @default.
- W2164421581 creator A5043906229 @default.
- W2164421581 date "1983-10-01" @default.
- W2164421581 modified "2023-09-23" @default.
- W2164421581 title "Partial Purification and Characterization of Two Enzymes Involved in Isovaleric Acid Synthesis in Clostridium bifermentans" @default.
- W2164421581 cites W1011585550 @default.
- W2164421581 cites W1515108352 @default.
- W2164421581 cites W1554702559 @default.
- W2164421581 cites W1565008906 @default.
- W2164421581 cites W1625660792 @default.
- W2164421581 cites W1837062668 @default.
- W2164421581 cites W2029170287 @default.
- W2164421581 cites W2050871726 @default.
- W2164421581 cites W2052284945 @default.
- W2164421581 cites W2052658229 @default.
- W2164421581 cites W2054556994 @default.
- W2164421581 cites W2054750277 @default.
- W2164421581 cites W2067571365 @default.
- W2164421581 cites W2100837269 @default.
- W2164421581 cites W2179100843 @default.
- W2164421581 cites W2196852987 @default.
- W2164421581 cites W2409105530 @default.
- W2164421581 cites W2893650296 @default.
- W2164421581 cites W3487810 @default.
- W2164421581 doi "https://doi.org/10.1099/00221287-129-10-3227" @default.
- W2164421581 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6655460" @default.
- W2164421581 hasPublicationYear "1983" @default.
- W2164421581 type Work @default.
- W2164421581 sameAs 2164421581 @default.
- W2164421581 citedByCount "4" @default.
- W2164421581 countsByYear W21644215812021 @default.
- W2164421581 countsByYear W21644215812023 @default.
- W2164421581 crossrefType "journal-article" @default.
- W2164421581 hasAuthorship W2164421581A5000714877 @default.
- W2164421581 hasAuthorship W2164421581A5043906229 @default.
- W2164421581 hasBestOaLocation W21644215811 @default.
- W2164421581 hasConcept C181199279 @default.
- W2164421581 hasConcept C185592680 @default.
- W2164421581 hasConcept C197957613 @default.
- W2164421581 hasConcept C2775859393 @default.
- W2164421581 hasConcept C2776580952 @default.
- W2164421581 hasConcept C2776834122 @default.
- W2164421581 hasConcept C2780859938 @default.
- W2164421581 hasConcept C28212737 @default.
- W2164421581 hasConcept C43617362 @default.
- W2164421581 hasConcept C515207424 @default.
- W2164421581 hasConcept C55493867 @default.
- W2164421581 hasConcept C68793091 @default.
- W2164421581 hasConceptScore W2164421581C181199279 @default.
- W2164421581 hasConceptScore W2164421581C185592680 @default.
- W2164421581 hasConceptScore W2164421581C197957613 @default.
- W2164421581 hasConceptScore W2164421581C2775859393 @default.
- W2164421581 hasConceptScore W2164421581C2776580952 @default.
- W2164421581 hasConceptScore W2164421581C2776834122 @default.
- W2164421581 hasConceptScore W2164421581C2780859938 @default.
- W2164421581 hasConceptScore W2164421581C28212737 @default.
- W2164421581 hasConceptScore W2164421581C43617362 @default.
- W2164421581 hasConceptScore W2164421581C515207424 @default.
- W2164421581 hasConceptScore W2164421581C55493867 @default.
- W2164421581 hasConceptScore W2164421581C68793091 @default.
- W2164421581 hasIssue "10" @default.
- W2164421581 hasLocation W21644215811 @default.
- W2164421581 hasLocation W21644215812 @default.
- W2164421581 hasOpenAccess W2164421581 @default.
- W2164421581 hasPrimaryLocation W21644215811 @default.
- W2164421581 hasRelatedWork W1542114658 @default.
- W2164421581 hasRelatedWork W2071806336 @default.
- W2164421581 hasRelatedWork W2078504056 @default.
- W2164421581 hasRelatedWork W2098689981 @default.
- W2164421581 hasRelatedWork W2206868927 @default.
- W2164421581 hasRelatedWork W2395085887 @default.
- W2164421581 hasRelatedWork W2400903474 @default.
- W2164421581 hasRelatedWork W2465072332 @default.
- W2164421581 hasRelatedWork W2564456489 @default.
- W2164421581 hasRelatedWork W2811032335 @default.
- W2164421581 hasVolume "129" @default.
- W2164421581 isParatext "false" @default.
- W2164421581 isRetracted "false" @default.
- W2164421581 magId "2164421581" @default.
- W2164421581 workType "article" @default.