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- W2164566356 abstract "The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein–protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames, proof-of-principle Y2H interaction screens were performed for the human neurodegenerative disease proteins huntingtin and ataxin-1. Using systematic controls for unspecific Y2H results and quantitative benchmarking, we identified and scored a large number of known and novel partner proteins for both huntingtin and ataxin-1. Moreover, we show that this parallelized screening procedure and the global inspection of Y2H interaction data are uniquely suited to define specific PPI patterns and their alteration by disease-causing mutations in huntingtin and ataxin-1. This approach takes advantage of the specificity and flexibility of DNA microarrays and of the existence of solid-related statistical methods for the analysis of DNA microarray data, and allows a quantitative approach toward interaction screens in human and in model organisms." @default.
- W2164566356 created "2016-06-24" @default.
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- W2164566356 date "2012-12-26" @default.
- W2164566356 modified "2023-10-17" @default.
- W2164566356 title "Development and application of a DNA microarray-based yeast two-hybrid system" @default.
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- W2164566356 doi "https://doi.org/10.1093/nar/gks1329" @default.
- W2164566356 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3561971" @default.
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- W2164566356 hasPublicationYear "2012" @default.
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