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• W2164677742 abstract "Although the biological conversion of nitrite to nitrate is a well-known process, studies of Nitrobacter populations are hindered by their physiological characteristics. This report describes a new method for detectingandcountingNitrobacterpopulationsinsituwiththePCR.Twoprimersfromthe16SrRNAgenewere used to generate a 397-bp fragment by amplification ofNitrobacterspecies DNA. No signal was detected from their phylogenetic neighbors or the common soil bacteria tested. Extraction and purification steps were optimized for minimal loss and maximal purity of soil DNA. The detection threshold and accuracy of the molecular method were determined from soil inoculated with 10, 10 2 ,or10 3 Nitrobacter hamburgensiscells per g of soil. Counts were also done by the most-probable-number (MPN)-Griess and fluorescent antibody methods. PCR had a lower detection threshold (10 2 Nitrobactercells per g of soil) than did the MPN-Griess or fluorescent antibody method. When PCR amplification was coupled with the MPN method, the counting rate reached 65 to 72% of inoculated Nitrobacter cells. Tested on nonsterile soil, this rapid procedure was proved efficient." @default.
• W2164677742 created "2016-06-24" @default.
• W2164677742 creator A5003727086 @default.
• W2164677742 creator A5074107124 @default.
• W2164677742 date "1995-01-01" @default.
• W2164677742 modified "2023-09-27" @default.
• W2164677742 title "Detection and Counting ofNitrobacterPopulations in Soil by PCR" @default.
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• W2164677742 hasPublicationYear "1995" @default.
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