FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2164678669> ?p ?o ?g. }

• W2164678669 abstract "ABSTRACT Cryptococcosis is an opportunistic infection due to the ubiquitous yeast Cryptococcus neoformans . This yeast interacts closely with innate immune cells, leading to various fates, including fungal persistence within cells, making possible the dissemination of the yeast cells with monocytes via a Trojan horse strategy. In humans, the natural history of the infection begins with primoinfection during childhood, which is followed by dormancy and, in some individuals, reactivation upon immunosuppression. To address the question of dormancy, we studied C. neoformans infection at the macrophage level ( in vitro H99-macrophage interaction) and at the organ level in a murine model of cryptococcosis. We analyzed the diversity of yeast adaptation to the host by characterizing several C. neoformans populations with new assays based on flow cytometry (quantitative flow cytometry, multispectral imaging flow cytometry, sorting), microscopy (dynamic imaging), and gene expression analysis. On the basis of parameters of multiplication and stress response, various populations of yeast cells were observed over time in vivo and in vitro . Cell sorting allowed the identification of a subpopulation that was less prone to grow under standard conditions than the other populations, with growth enhanced by the addition of serum. Gene expression analysis revealed that this population had specific metabolic characteristics that could reflect dormancy. Our data suggest that dormant yeast cells could exist in vitro and in vivo . C. neoformans exhibits a huge plasticity and adaptation to hosts that deserves further study. In vitro generation of dormant cells is now the main challenge to overcome the limited number of yeast cells recovered in our models. IMPORTANCE Cryptococcus neoformans is a sugar-coated unicellular fungus that interacts closely with various cells and organisms, including amoebas, nematodes, and immune cells of mammals. This yeast is able to proliferate and survive in the intracellular environment. C. neoformans causes cryptococcosis, and yeast dormancy in humans has been suggested on the basis of epidemiological evidence obtained years ago. By studying an in vitro model of yeast-macrophage interaction and murine models of cryptococcosis, we observed that yeast cells evolve in heterogeneous populations during infection on the basis of global metabolic activity. We compared the growth ability and gene expression of yeast cells belonging to various populations in those two models. We eventually found a population of yeast cells with low metabolism that fit some of the criteria for dormant cells. This paves the way for further characterization of dormancy in C. neoformans ." @default.
• W2164678669 created "2016-06-24" @default.
• W2164678669 creator A5042541722 @default.
• W2164678669 creator A5055184594 @default.
• W2164678669 creator A5059831856 @default.
• W2164678669 creator A5075168686 @default.
• W2164678669 date "2015-05-01" @default.
• W2164678669 modified "2023-10-01" @default.
• W2164678669 title "Cryptococcus neoformans Host Adaptation: Toward Biological Evidence of Dormancy" @default.
• W2164678669 cites W1483287668 @default.
• W2164678669 cites W1512904903 @default.
• W2164678669 cites W1754630493 @default.
• W2164678669 cites W1756749354 @default.
• W2164678669 cites W1816895742 @default.
• W2164678669 cites W1824251371 @default.
• W2164678669 cites W1860530067 @default.
• W2164678669 cites W1965580171 @default.
• W2164678669 cites W1970417127 @default.
• W2164678669 cites W1975401101 @default.
• W2164678669 cites W1978483995 @default.
• W2164678669 cites W1980954479 @default.
• W2164678669 cites W1991631212 @default.
• W2164678669 cites W1992872085 @default.
• W2164678669 cites W1994648408 @default.
• W2164678669 cites W2003105635 @default.
• W2164678669 cites W2004173797 @default.
• W2164678669 cites W2014442978 @default.
• W2164678669 cites W2018055448 @default.
• W2164678669 cites W2030574963 @default.
• W2164678669 cites W2031984913 @default.
• W2164678669 cites W2033679946 @default.
• W2164678669 cites W2035341197 @default.
• W2164678669 cites W2035772703 @default.
• W2164678669 cites W2050400409 @default.
• W2164678669 cites W2055416073 @default.
• W2164678669 cites W2056573634 @default.
• W2164678669 cites W2058779017 @default.
• W2164678669 cites W2063779731 @default.
• W2164678669 cites W2064009861 @default.
• W2164678669 cites W2066050762 @default.
• W2164678669 cites W2068790641 @default.
• W2164678669 cites W2072853512 @default.
• W2164678669 cites W2073363331 @default.
• W2164678669 cites W2094943390 @default.
• W2164678669 cites W2095558727 @default.
• W2164678669 cites W2096355838 @default.
• W2164678669 cites W2096513608 @default.
• W2164678669 cites W2097423540 @default.
• W2164678669 cites W2099142461 @default.
• W2164678669 cites W2100317350 @default.
• W2164678669 cites W2104014765 @default.
• W2164678669 cites W2104565935 @default.
• W2164678669 cites W2108244474 @default.
• W2164678669 cites W2108531991 @default.
• W2164678669 cites W2109118083 @default.
• W2164678669 cites W2110926912 @default.
• W2164678669 cites W2111345804 @default.
• W2164678669 cites W2111715441 @default.
• W2164678669 cites W2112452073 @default.
• W2164678669 cites W2116900936 @default.
• W2164678669 cites W2117474926 @default.
• W2164678669 cites W2120739562 @default.
• W2164678669 cites W2128169528 @default.
• W2164678669 cites W2130005442 @default.
• W2164678669 cites W2132011921 @default.
• W2164678669 cites W2134384685 @default.
• W2164678669 cites W2136651521 @default.
• W2164678669 cites W2140101678 @default.
• W2164678669 cites W2140810025 @default.
• W2164678669 cites W2143703444 @default.
• W2164678669 cites W2145443134 @default.
• W2164678669 cites W2149856975 @default.
• W2164678669 cites W2151607863 @default.
• W2164678669 cites W2152206032 @default.
• W2164678669 cites W2154320236 @default.
• W2164678669 cites W2154604278 @default.
• W2164678669 cites W2158372574 @default.
• W2164678669 cites W2159046810 @default.
• W2164678669 cites W2159678218 @default.
• W2164678669 cites W2160338568 @default.
• W2164678669 cites W2162644900 @default.
• W2164678669 cites W2162897958 @default.
• W2164678669 cites W2165278359 @default.
• W2164678669 cites W2323193510 @default.
• W2164678669 cites W2418512045 @default.
• W2164678669 cites W4236447205 @default.
• W2164678669 cites W45534775 @default.
• W2164678669 doi "https://doi.org/10.1128/mbio.02580-14" @default.
• W2164678669 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4453510" @default.
• W2164678669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25827423" @default.
• W2164678669 hasPublicationYear "2015" @default.
• W2164678669 type Work @default.
• W2164678669 sameAs 2164678669 @default.
• W2164678669 citedByCount "87" @default.
• W2164678669 countsByYear W21646786692014 @default.
• W2164678669 countsByYear W21646786692015 @default.
• W2164678669 countsByYear W21646786692016 @default.
• W2164678669 countsByYear W21646786692017 @default.
• W2164678669 countsByYear W21646786692018 @default.
• W2164678669 countsByYear W21646786692019 @default.

• next