Matches in SemOpenAlex for { <https://semopenalex.org/work/W2164751102> ?p ?o ?g. }
- W2164751102 endingPage "4564" @default.
- W2164751102 startingPage "4564" @default.
- W2164751102 abstract "We have developed a microfluidic device that enhances the sensitivity of protein immunoassays by preconcentrating the protein sample using isotachophoresis (ITP). Two approaches were followed to study the sensitivity gain achieved that way. The first approach was using antibody-coated magnetic beads loaded into a microchannel to capture the proteins within the ITP sample zone. The second was to directly bind the antibodies to the microchannel surface. The use of ITP increased the sensitivity of both the direct and the bead-based immunoassay and lowered the model protein's detection limit. Our setup also uses electrokinetic injection for sample charging, thereby sparing the need for integrating mechanical components such as pumps or valves and reducing the complexity of the setup. This work demonstrates the feasibility of using ITP as a preconcentration mechanism of proteins in immunoassays. The protein was concentrated by a factor of 100 and the assay's limit of detection was in the picomolar range. ITP can be applied to any sample of mixed content, separating and preconcentrating the desired analyte before it reaches the detection region with immobilized antibodies, without the need for separating and concentrating the sample in an independent step." @default.
- W2164751102 created "2016-06-24" @default.
- W2164751102 creator A5015800902 @default.
- W2164751102 creator A5030095106 @default.
- W2164751102 creator A5039743851 @default.
- W2164751102 creator A5040615064 @default.
- W2164751102 date "2014-06-18" @default.
- W2164751102 modified "2023-10-03" @default.
- W2164751102 title "Increasing the sensitivity of microfluidics based immunoassays using isotachophoresis" @default.
- W2164751102 cites W1976819923 @default.
- W2164751102 cites W1977458643 @default.
- W2164751102 cites W1985803069 @default.
- W2164751102 cites W1989104753 @default.
- W2164751102 cites W1995768430 @default.
- W2164751102 cites W1996334281 @default.
- W2164751102 cites W2002102397 @default.
- W2164751102 cites W2003054850 @default.
- W2164751102 cites W2003062413 @default.
- W2164751102 cites W2009058878 @default.
- W2164751102 cites W2014554686 @default.
- W2164751102 cites W2016790437 @default.
- W2164751102 cites W2019354697 @default.
- W2164751102 cites W2025163220 @default.
- W2164751102 cites W2035882743 @default.
- W2164751102 cites W2038887100 @default.
- W2164751102 cites W2042301048 @default.
- W2164751102 cites W2043794246 @default.
- W2164751102 cites W2062047349 @default.
- W2164751102 cites W2071838189 @default.
- W2164751102 cites W2075445155 @default.
- W2164751102 cites W2083219397 @default.
- W2164751102 cites W2084405918 @default.
- W2164751102 cites W2094532724 @default.
- W2164751102 cites W2098633866 @default.
- W2164751102 cites W2101335388 @default.
- W2164751102 cites W2109311842 @default.
- W2164751102 cites W2109950581 @default.
- W2164751102 cites W2120967142 @default.
- W2164751102 cites W2123461766 @default.
- W2164751102 cites W2134745814 @default.
- W2164751102 cites W2141071099 @default.
- W2164751102 cites W2141089321 @default.
- W2164751102 cites W2148518048 @default.
- W2164751102 cites W2151347407 @default.
- W2164751102 cites W2158055717 @default.
- W2164751102 cites W2159337830 @default.
- W2164751102 cites W2314136438 @default.
- W2164751102 cites W2315312847 @default.
- W2164751102 doi "https://doi.org/10.1039/c4an00545g" @default.
- W2164751102 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25028696" @default.
- W2164751102 hasPublicationYear "2014" @default.
- W2164751102 type Work @default.
- W2164751102 sameAs 2164751102 @default.
- W2164751102 citedByCount "17" @default.
- W2164751102 countsByYear W21647511022014 @default.
- W2164751102 countsByYear W21647511022015 @default.
- W2164751102 countsByYear W21647511022016 @default.
- W2164751102 countsByYear W21647511022017 @default.
- W2164751102 countsByYear W21647511022018 @default.
- W2164751102 countsByYear W21647511022020 @default.
- W2164751102 countsByYear W21647511022021 @default.
- W2164751102 countsByYear W21647511022022 @default.
- W2164751102 countsByYear W21647511022023 @default.
- W2164751102 crossrefType "journal-article" @default.
- W2164751102 hasAuthorship W2164751102A5015800902 @default.
- W2164751102 hasAuthorship W2164751102A5030095106 @default.
- W2164751102 hasAuthorship W2164751102A5039743851 @default.
- W2164751102 hasAuthorship W2164751102A5040615064 @default.
- W2164751102 hasConcept C10390740 @default.
- W2164751102 hasConcept C119128265 @default.
- W2164751102 hasConcept C127413603 @default.
- W2164751102 hasConcept C147789679 @default.
- W2164751102 hasConcept C159654299 @default.
- W2164751102 hasConcept C171250308 @default.
- W2164751102 hasConcept C17525397 @default.
- W2164751102 hasConcept C185592680 @default.
- W2164751102 hasConcept C186293402 @default.
- W2164751102 hasConcept C192562407 @default.
- W2164751102 hasConcept C203014093 @default.
- W2164751102 hasConcept C21200559 @default.
- W2164751102 hasConcept C24326235 @default.
- W2164751102 hasConcept C27703432 @default.
- W2164751102 hasConcept C2780420688 @default.
- W2164751102 hasConcept C3019566124 @default.
- W2164751102 hasConcept C43617362 @default.
- W2164751102 hasConcept C63662833 @default.
- W2164751102 hasConcept C68801617 @default.
- W2164751102 hasConcept C8673954 @default.
- W2164751102 hasConcept C86803240 @default.
- W2164751102 hasConceptScore W2164751102C10390740 @default.
- W2164751102 hasConceptScore W2164751102C119128265 @default.
- W2164751102 hasConceptScore W2164751102C127413603 @default.
- W2164751102 hasConceptScore W2164751102C147789679 @default.
- W2164751102 hasConceptScore W2164751102C159654299 @default.
- W2164751102 hasConceptScore W2164751102C171250308 @default.
- W2164751102 hasConceptScore W2164751102C17525397 @default.
- W2164751102 hasConceptScore W2164751102C185592680 @default.
- W2164751102 hasConceptScore W2164751102C186293402 @default.