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• W2164802419 abstract "Established cell lines have long been used for in vitro studies of tumor biology, enabling investigators to control growth conditions and to draw important conclusions about the oncogenic microenvironment. However, gene expression behavior in cultured cells may not always reflect the actual in vivo scenario, and analysis derived from such experiments should take into consideration the existing differences between the two environments. We used suppression subtractive hybridization to study transcriptional changes elicited after oncogene transformation and cell line establishment. We found that transcriptional changes elicited in cultured cell lines are in fact representative of late events, and they do not occur early after oncogene transfection or activation. We also determined that a fraction of the transcriptional changes is oncogene specific, whereas other changes are shared between two or more different oncogenes." @default.
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• W2164802419 date "2004-09-30" @default.
• W2164802419 modified "2023-09-27" @default.
• W2164802419 title "Oncogene-induced transcriptional patterns in established cell lines as a model for in vitro analysis of tumor biology." @default.
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• W2164802419 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15614731" @default.
• W2164802419 hasPublicationYear "2004" @default.
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