FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2164834056> ?p ?o ?g. }

• W2164834056 endingPage "1435" @default.
• W2164834056 startingPage "1422" @default.
• W2164834056 abstract "Abstract: Extracellular levels of endogenous serotonin (5-HT) and its major metabolite, 5-hydroxyindoleacetic acid (5-HIAA), were measured in the caudate-putamen of anesthetized and awake rats using intracerebral microdialysis coupled to HPLC with fluorimetric detection. A dialysis probe (of the loop type) was perfused with Ringer solution at 2 μl/min, and samples collected every 30 or 60 min. Basal indole levels were followed for up to 4 days in both intact and 5,7-dihydroxytryptamine (5,7-DHT) lesioned animals. Immediately after the probe implantation, the striatal 5-HT levels were about 10 times higher than the steady-state levels that were reached after 7-8 h of perfusion. The steady-state baseline levels, which amounted to 22.5 fmol/30 min sampling time, remained stable for 4 days. In 5,7-DHT-denervated animals, the steady-state levels of 5-HT, measured during the second day after probe implantation, were below the limit of detection (<10 fmol/60 min). However, during the first 6h post-implantation, the 5-HT output was as high as in intact animals, which suggests that the high 5-HT levels recovered in association with probe implantation were blood-derived. As a consequence, all other experiments were started after a delay of at least 12 h after implantation of the dialysis probe. In awake, freely moving animals, the steady-state 5-HT levels were about 60% higher than in halothane-anesthetized animals, whereas 5-HIAA was unaffected by anesthesia. KCI (60 and 100 mM) added to the perfusion fluid produced a sharp increase in 5-HT output that was eight-fold at the 60 mM concentration and 21-fold at the 100 mM concentration. In contrast, 5-HIAA output dropped by 43 and 54%, respectively. In 5,7-DHT-lesioned animals, the KCl-evoked (100 mM) release represented less than 5% of the peak values obtained for the intact striata. Omission of Ca2+ from the perfusion fluid resulted in a 70% reduction in baseline 5-HT output, whereas the 5-HIAA levels remained unchanged. High concentrations of tetrodotoxin (TTX) added to the perfusion medium (5-50 μM) resulted in quite variable results. At a lower concentration (1 μM), however, TTX produceda 50% reduction in baseline 5-HT release, whereas the 5-HIAA output remained unchanged. The 5-HT reuptake blocker, indalpine, increased the extracellular levels of 5-HT sixfold when added to the perfusion medium (1 μM), and threefold when given intraperitoneally (5 mg/kg). By contrast, the 5-HIAA level remained unaffected during indalpine infusion. Application of TTX (1 μM) under simultaneous 5-HT uptake blockade induced a decrease in 5-HT output by 62–71%. p-Chloroamphetamine (2.5 mg/kg, i.p.) induced a 12-fold increase in 5-HT release and reduced the 5-HIAA output by about 50%. The p-chloroamphetamine-induced increase in 5-HT release was 10 times lower in the 5,7-DHT-denervated striatum. Pargyline (75 mg/kg, i.p.) increased the extracellular levels of 5-HT 11-fold within 6 h, and reduced the 5-HIAA levels by 80%. The 5-HT receptor agonist, 5-methoxy-N,N-dimethyltryptamine (1 mg/kg, i.p.), produced an immediate reduction of about 50% in 5-HT release and a small (11 %) decrease in 5-HIAA output. It is concluded (1) that intracerebral microdialysis coupled to HPLC with fluorimetric detection provides a useful method for the study of extracellular 5-HT and 5-HIAA levels; (2) that steady-state levels of 5-HT and 5-HIAA recovered in the dialysis perfusate are neuronally derived, but these steady-state levels are reached only after a minimum of 7–8 h after probe implantation; (3) that changes in striatal extracellular levels of 5-HT are closely related to changes in serotonergic synaptic activity; and (4) that extracellular levels of 5-HIAA are a poor indicator of synaptic activity, and instead primarily reflect intraneuronal metabolism." @default.
• W2164834056 created "2016-06-24" @default.
• W2164834056 creator A5047056131 @default.
• W2164834056 creator A5047762447 @default.
• W2164834056 creator A5058028504 @default.
• W2164834056 creator A5089555188 @default.
• W2164834056 date "1988-11-01" @default.
• W2164834056 modified "2023-10-11" @default.
• W2164834056 title "Endogenous Release of Neuronal Serotonin and 5-Hydroxyindoleacetic Acid in the Caudate-Putamen of the Rat as Revealed by Intracerebral Dialysis Coupled to High-Performance Liquid Chromatography with Fluorimetric Detection" @default.
• W2164834056 cites W1521916566 @default.
• W2164834056 cites W1964146392 @default.
• W2164834056 cites W1964412055 @default.
• W2164834056 cites W1966750852 @default.
• W2164834056 cites W1968957681 @default.
• W2164834056 cites W1971733225 @default.
• W2164834056 cites W1973527554 @default.
• W2164834056 cites W1974869837 @default.
• W2164834056 cites W1975744305 @default.
• W2164834056 cites W1979446417 @default.
• W2164834056 cites W1981119192 @default.
• W2164834056 cites W1981783120 @default.
• W2164834056 cites W1984508889 @default.
• W2164834056 cites W1985288617 @default.
• W2164834056 cites W1987097394 @default.
• W2164834056 cites W1989796420 @default.
• W2164834056 cites W1989901803 @default.
• W2164834056 cites W1991485877 @default.
• W2164834056 cites W2003661602 @default.
• W2164834056 cites W2003954063 @default.
• W2164834056 cites W2004162941 @default.
• W2164834056 cites W2004895078 @default.
• W2164834056 cites W2006370215 @default.
• W2164834056 cites W2007013230 @default.
• W2164834056 cites W2008574754 @default.
• W2164834056 cites W2009768370 @default.
• W2164834056 cites W2011798603 @default.
• W2164834056 cites W2015863527 @default.
• W2164834056 cites W2021365267 @default.
• W2164834056 cites W2021894022 @default.
• W2164834056 cites W2023746965 @default.
• W2164834056 cites W2026189743 @default.
• W2164834056 cites W2028596709 @default.
• W2164834056 cites W2032479084 @default.
• W2164834056 cites W2034107056 @default.
• W2164834056 cites W2034146177 @default.
• W2164834056 cites W2034371383 @default.
• W2164834056 cites W2038783305 @default.
• W2164834056 cites W2040468588 @default.
• W2164834056 cites W2041149109 @default.
• W2164834056 cites W2041763391 @default.
• W2164834056 cites W2049062911 @default.
• W2164834056 cites W2051525768 @default.
• W2164834056 cites W2055030165 @default.
• W2164834056 cites W2055782174 @default.
• W2164834056 cites W2060345670 @default.
• W2164834056 cites W2063277383 @default.
• W2164834056 cites W2066482939 @default.
• W2164834056 cites W2067847619 @default.
• W2164834056 cites W2068535052 @default.
• W2164834056 cites W2070146489 @default.
• W2164834056 cites W2072435070 @default.
• W2164834056 cites W2073723494 @default.
• W2164834056 cites W2077021584 @default.
• W2164834056 cites W2079347869 @default.
• W2164834056 cites W2082072754 @default.
• W2164834056 cites W2082147150 @default.
• W2164834056 cites W2091347056 @default.
• W2164834056 cites W2092279314 @default.
• W2164834056 cites W2093037732 @default.
• W2164834056 cites W2100869641 @default.
• W2164834056 cites W2111125844 @default.
• W2164834056 cites W2132194608 @default.
• W2164834056 cites W2145295139 @default.
• W2164834056 cites W2148801102 @default.
• W2164834056 cites W2153823370 @default.
• W2164834056 cites W2498584891 @default.
• W2164834056 cites W285648030 @default.
• W2164834056 doi "https://doi.org/10.1111/j.1471-4159.1988.tb01107.x" @default.
• W2164834056 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2459309" @default.
• W2164834056 hasPublicationYear "1988" @default.
• W2164834056 type Work @default.
• W2164834056 sameAs 2164834056 @default.
• W2164834056 citedByCount "234" @default.
• W2164834056 countsByYear W21648340562012 @default.
• W2164834056 countsByYear W21648340562013 @default.
• W2164834056 countsByYear W21648340562014 @default.
• W2164834056 countsByYear W21648340562015 @default.
• W2164834056 countsByYear W21648340562018 @default.
• W2164834056 countsByYear W21648340562020 @default.
• W2164834056 countsByYear W21648340562021 @default.
• W2164834056 countsByYear W21648340562022 @default.
• W2164834056 countsByYear W21648340562023 @default.
• W2164834056 crossrefType "journal-article" @default.
• W2164834056 hasAuthorship W2164834056A5047056131 @default.
• W2164834056 hasAuthorship W2164834056A5047762447 @default.
• W2164834056 hasAuthorship W2164834056A5058028504 @default.
• W2164834056 hasAuthorship W2164834056A5089555188 @default.
• W2164834056 hasConcept C126322002 @default.

• next